草业学报2024，Vol.33Issue(8)：170-180,11.DOI:10.11686/cyxb2023392
四个品种红三叶种带真菌多样性研究
Diversity of seed-borne fungi on four Trifolium pratense cultivars
摘要
Abstract
Red clover (Trifolium pratense) is an important leguminous forage and green manure crop.However,seed-borne fungi severely affect the seed quality of red clover,and are among the major limiting factors for red clover production.In this study,four varieties of red clover were selected to investigate the species and diversity of seed-borne fungi using indoor isolation and high-throughput sequencing methods.A total of 26 species from 15 genera of seedborne fungi were identified from the four tested varieties,including 14 species from nine genera in T.pratense (cv.Minshan),seven species from five genera in T.pratense (cv.Ruide),nine species from nine genera in T.pratense (cv.VNS),and 11 species from seven genera in T.pratense (cv.Honglong).High-throughput sequencing of fungi from the seed samples of the four red clover varieties identified a total of 87 species from 30 genera,including Cladosporium,Alternaria,Penicillium,Aspergillus,Stemphylium,Sporobolomyces,Filobasidium,Udeniomyces,and other genera.Among them,several species of the genera Stemphylium,Alternaria,Cladosporium,Acremonium,Botrytis,Fusarium,and Erysiphe were identified as potential pathogens.Alpha and beta diversity analyses revealed significant differences in fungal community composition and abundance among the red clover varieties,with T.pratense (cv.Minshan) exhibiting higher diversity of seed-borne fungi compared with that of the other three red clover varieties.关键词
红三叶/种带真菌/高通量测序/多样性Key words
Trifolium pratense/seed-borne fungi/high-throughput sequencing/diversity引用本文复制引用
郑荣春,南志标,段廷玉..四个品种红三叶种带真菌多样性研究[J].草业学报,2024,33(8):170-180,11.基金项目
国家绿肥技术产业体系(CARS-22)资助. （CARS-22）
