茶叶学报2024，Vol.65Issue(3)：11-33,23.DOI:10.20045/j.cnki.issn.2096-0220.2024.03.002
基于金观音(Camellia sinensis)单体型基因组CsUGT等位基因的鉴定及表达
Identification and Expression of CsUGT Alleles Based on Haplotype-resolved Genomes of Camellia sinensis Jinguanyin
摘要
Abstract
[Objective]Key genes responsible of generating uridine diphosphate glycosyltransferase(UGT)that cataly-zes the glycosylation of the secondary metabolism associated with the stress response of Camellia sinensiswere identified,and expressions under stress analyzed to decipher the aroma formation of oolong tea in processing.[Method]Based on the haplotype-resolved genomes of C.sinensis Jinguanyin,CsUGT alleles were analyzed by bioinformatics and RT-PCR.Expressions of CsUGT at 0,3,6,12,and 24 h under stresses of low temperature(4℃),drought(10%PEG-6000 solution),or hormone(100 μmol·L-1 MeJA solution)as well as in the young leaves,withering,post-1st turning-over,post-2nd turning-over,post-3rd turning-over,and before firing in the processing stages of Jinguanyin oolong tea were determined.[Result]The 142 CsUGT allele pairs identified from the haplotype-resolved genomes were mostly in the chloroplasts and could be divided into 5 subfamilies based on the evolutionary tree.These alleles were conserved and similar in structure when came from the same parent.Expressions of most of the alleles were tissue specific playing an important role in different organs of the tea plant.The expressions of CsUGT002,CsUGT047,and CsUGT091 peaked at 6h under MeJA treatment,while some peaked in 3 h under cold stress and 24 h under drought conditions.The expression of CsUGT115 was significantly upregulated during processing,while some others peaked at the 3rd turning-over stage.[Conclusion]Most CsUGTs were acidic,hydrophilic,and found in the chloroplast.Expressions of CsUGT alleles were tissue specific.Some CsUGTs associated with the aroma synthesis were activated by the stresses exerted by the tea processing.关键词
茶树/金观音/尿苷二磷酸糖基转移酶/等位基因/逆境胁迫/香气/生物信息学Key words
Camellia sinensis/Jinguanyin/UDP-glycosyltransferase(UGT)/allele/adversity stress/aroma/bioinformatics分类
轻工纺织引用本文复制引用
雷小梅,张宇航,谷梦雅,郑玉成,叶乃兴..基于金观音(Camellia sinensis)单体型基因组CsUGT等位基因的鉴定及表达[J].茶叶学报,2024,65(3):11-33,23.基金项目
中国乌龙茶产业协同创新中心开放基金(2024W01) （2024W01）
福建张天福茶叶发展基金会科技创新基金(FJZTF01). （FJZTF01）
