茶叶学报2024，Vol.65Issue(3)：34-46,13.DOI:10.20045/j.cnki.issn.2096-0220.2024.03.003
茶树PAL、C4H与4CL基因家族的全基因组鉴定及表达分析
Identification and Expressions of PAL,C4H,and 4CL Families in Tea Plant
摘要
Abstract
[Objective]Genetic characteristics of PAL,C4H,and 4CL family members in tea were studied to examine the connection between these genes and abundance of phenylethanol primrose glycoside(PPG)in the plant.[Method]Using bioinformatics and transcriptome sequencing techniques,the distribution,physicochemical properties,gene structure,phylogenetic relationships,promoter cis-acting elements,and expression characteristics of CsPAL,CsC4H,and Cs4CLs in the genome of tea plants were analyzed.Expressions of these genes in the shoots of low-and high-glycoside teas,which differed significantly in PPG content,were compared.[Result]Seven CsPALs,4 CsC4Hs,and 4 Cs4CLs were found in 9 chromosomes of tea plants.All CsPALs had only one intron,while all CsC4Hs other than CsC4H4 had two and all Cs4CLs had multiple introns.Phylogenetically,they closely related to PAL,C4H,and 4CL of Populus trichocarpa with a similarity in structure and conserved motifs.The promoter regions of CsPALs,CsC4Hs,and Cs4CLs contained elements responding to stresses,growth,development,and presence of hormones.The tissue specific gene expression could be induced by exposure to hormones,low temperature,drought,or high salinity.Significantly differentiated,CsPAL7 was highly expressed in the high-glycoside teas than the low-glycoside teas,but CsC4H4 and Cs4CL3 were the opposite.The varied expression patterns might indicate different roles these families played in the PPG formation in tea plants.[Conclusion]The information obtained on CsPALs,CsC4Hs,and Cs4CLs would be of value for the understanding of the biological functions of these genes and breeding new tea varieties.关键词
茶树/PAL基因家族/C4H基因家族/4CL基因家族/表达分析Key words
Tea/PALs/C4Hs/4CLs/expression analysis分类
农业科技引用本文复制引用
张力岚,杨军,王让剑..茶树PAL、C4H与4CL基因家族的全基因组鉴定及表达分析[J].茶叶学报,2024,65(3):34-46,13.基金项目
福建省属公益类科研院所基本科研专项——竞争性项目(2023R1090) （2023R1090）
农业高质量发展超越"5511"协同创新工程(XTCXGC2021004). （XTCXGC2021004）
