茶叶学报2024，Vol.65Issue(3)：57-66,10.DOI:10.20045/j.cnki.issn.2096-0220.2024.03.005
QuEChERS-GC-MS法快速检测茶叶中的芳樟醇和alpha-紫罗兰酮
A Rapid QuEChERS-GC-MS Assay for Linalool and Alpha-ionone Determinations in Tea
游佩琼1
作者信息
- 1. 福建省产品质量检验研究院,福建 福州 350002
- 折叠
摘要
Abstract
[Objective]An assay to detect the aromatic linalool and alpha-ionone in tea applying gas chromatography-mass spectrometry(GC-MS)was developed.[Method]Tea samples were firstly extracted by acetonitrile to collect the supernatant for the QuEChERS purification on a column containing 200 mg of PSA,200 mg of CB,and 400 mg of anhydrous sodium sulfate prior to GC-MS analysis.Under the ion monitoring mode(SIM),aromatic compounds were quantifiably determined with external standards.[Result]The assay detection limit on linalool was 0.020 mg·kg-1 and that on alpha-ionone 0.004 mg·kg-1;while the quantification limit was 0.080 mg·kg-1 on linalool and 0.010 mg·kg-1 on alpha-ionone.The measurement curves on the two aromatherapy substances were linear in concentrations of 5.0-2000.0 μg·L-1 with a greater than 0.999 correlation coefficient(R2).On the blank control sample of tea spiked with 5.0,10.0,and 1000.0 μg·L-1 of standard linalool and alpha-ionone,the assay showed an average recovery of 88.23%-106.11%with a relative standard deviation(RSD)of 1.98%-3.25%(n=6).[Conclusion]The newly developed method was rapid with a running time of only 16.8 m and highly reproducible,accurate,and sensitive results.It was deemed applicable for rapid detection of linalool and alpha-ionone in tea.关键词
茶叶/香气物质/QuEChERS/GC-MS/芳樟醇/alpha-紫罗兰酮Key words
Tea/aroma/QuEChERS/GC-MS/linalool/alpha-ionone分类
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游佩琼..QuEChERS-GC-MS法快速检测茶叶中的芳樟醇和alpha-紫罗兰酮[J].茶叶学报,2024,65(3):57-66,10.
