动物医学进展2024，Vol.45Issue(9)：7-12,6.
猪伪狂犬病病毒gG、gE和TK基因多重PCR检测方法的建立及应用
Establishment and Application of A multiplex PCR Assay for Detection of gG,gE and TK Gene of Porcine Pseudorabies Virus
摘要
Abstract
The aim of the study was to rapidly and efficiently diagnose porcine pseudorabies virus(PRV)and to distinguish PRV from vaccine strains.Nine pairs of specific primers were synthesized for genome sequen-cing of gG、gE and TK genes.The reaction conditions of this multiplex PCR were obtained after optimiza-tion,and specificity,sensitivity,reproducibility of multiplex PCR were determination.Results showed that the multiplex PCR could simultaneously detecting gG、gE and TK genes,and the limit was 2.5 ×10-5 ng/μL.PCV2,RRSV,PEDV,and Hps were not amplified by this method.Among the 53 samples from por-cine,7 samples were positive based on the multiplex PCR for gE genes,which indicated that these 7 sam-ples were infected by wild-type virus.These positive samples were the same samples based on single PCR.The concordance of the results based on two methods was 100％.In conclusion,the multiplex PCR could be used for the detection and epidemic logical investigation for pseudorabies virus.关键词
猪伪狂犬病病毒/gG基因/gE基因/TK基因/多重PCRKey words
Porcine pseudorabies virus/gG/gE/TK/multiplex PCR分类
畜牧业引用本文复制引用
吴晓敏,陈润山,李华明,项维,徐梦然,杨荣荣,雷连成,张付贤..猪伪狂犬病病毒gG、gE和TK基因多重PCR检测方法的建立及应用[J].动物医学进展,2024,45(9):7-12,6.基金项目
国家科技部"十四五"重点研发计划项目(2021YFD1800405) （2021YFD1800405）
国家自然科学基金项目(32072823) （32072823）
