动物医学进展2024，Vol.45Issue(9)：12-19,8.
猫泛白细胞减少症病毒YX01株的分离鉴定及致病性分析
Isolation,Identification and Pathogenicity Analysis of Feline Panleukopenia Virus Strain YX01
摘要
Abstract
This study isolated a strain of feline panleukopenia virus(FPV)from tissue samples of a suspec-ted FPV-infected cat with decreased white blood cells using F81 cells,named the FPV YX01 strain.The bi-ological characteristics of the FPV YX01 strain were analyzed through electron microscopy,serum aggluti-nation test,and treatment with chemical reagents(20％ether and 0.1 mol/L HCl).The genetic evolution of the FPV YX01 strain was analyzed based on the VP2 gene sequence.The pathogenicity of the isolated strain was evaluated through animal regression experiments.The results showed that the FPV YX01 strain caused typical pathological changes in F81 cells after infection,such as shrinkage,stretching,detachment,and slow growth.Under electron microscopy,FPV YX01 viral particles were shown to be round,without a membra-nous structure on the surface,with a diameter of about 25 nm and could be recognized by FPV-specific anti-bodies.Genetic evolution analysis found that the key amino acid sites determining FPV coagulation activity and virulence in the VP2 protein of the YX01 strain had not undergone mutations,and the YX01 strain was highly homologous to the Italian EU498695 strain.Animal regression experiments showed that infection with the YX01 strain caused typical clinical symptoms of feline panleukopenia,such as decreased appetite,diarrhea,biphasic fever,intestinal flatulence,severe reduction in white blood cell count,and liver bleeding.Pathological sections also showed bleeding and inflammatory cell infiltration in liver and intestinal tissues.In summary,this study isolated a highly pathogenic FPV epidemic strain YX01,providing a material basis for the study of FPV epidemiology,the development of diagnostic reagents,and the preparation of thera-peutic antibodies.关键词
猫泛白细胞减少症病毒/分离鉴定/生物学特性/致病性Key words
Feline panleukopenia virus/isolation and identification/biological characteristics/pathogenicity分类
农业科技引用本文复制引用
马建伟,徐义刚,李园,付展鸿,钟林翰,韩言言,王以欣,姜晓霞,孙晓波,王美,宋厚辉..猫泛白细胞减少症病毒YX01株的分离鉴定及致病性分析[J].动物医学进展,2024,45(9):12-19,8.基金项目
浙江农林大学科研发展基金项目(2023LFR007) （2023LFR007）
浙江农林大学大学生科研训练项目(2023KX089) （2023KX089）
