猫泛白细胞减少症病毒YX01株的分离鉴定及致病性分析OA北大核心CSTPCD

This study isolated a strain of feline panleukopenia virus(FPV)from tissue samples of a suspec-ted FPV-infected cat with decreased white blood cells using F81 cells,named the FPV YX01 strain.The bi-ological characteristics of the FPV YX01 strain were analyzed through electron microscopy,serum aggluti-nation test,and treatment with chemical reagents(20％ether and 0.1 mol/L HCl).The genetic evolution of the FPV YX01 strain was analyzed based on the VP2 gene sequence.The pathogenicity of the isolated strain was evaluated through animal regression experiments.The results showed that the FPV YX01 strain caused typical pathological changes in F81 cells after infection,such as shrinkage,stretching,detachment,and slow growth.Under electron microscopy,FPV YX01 viral particles were shown to be round,without a membra-nous structure on the surface,with a diameter of about 25 nm and could be recognized by FPV-specific anti-bodies.Genetic evolution analysis found that the key amino acid sites determining FPV coagulation activity and virulence in the VP2 protein of the YX01 strain had not undergone mutations,and the YX01 strain was highly homologous to the Italian EU498695 strain.Animal regression experiments showed that infection with the YX01 strain caused typical clinical symptoms of feline panleukopenia,such as decreased appetite,diarrhea,biphasic fever,intestinal flatulence,severe reduction in white blood cell count,and liver bleeding.Pathological sections also showed bleeding and inflammatory cell infiltration in liver and intestinal tissues.In summary,this study isolated a highly pathogenic FPV epidemic strain YX01,providing a material basis for the study of FPV epidemiology,the development of diagnostic reagents,and the preparation of thera-peutic antibodies.