3株鸡源沙门氏菌的耐药性和致病性分析OA北大核心CSTPCD

The aim of this study is identify suspected Salmonella isolated from three laying hen farms in Hebei province,China,Samples were subjected to biochemical identification,PCR identification,drug sensi-tivity testing,PCR identification of toxing genes,PCR identification of drug resistance genes,chicken path-ogenicity tests.The results showed that the resistance rate of the isolates to amoxicillin was up to 100％,and they were resistant to at least four antimicrobials simultaneously;The isolates carried a total of 8 kinds of drug resistance genes,among which the detection rates of floR,sul3 and blaOXA-1 were 100％;The isolates carried 10 kinds of virulence genes,among which the detection rates of sip A,sipC,invH,mgtC and stnP1 were the highest at 100％;There were deaths in all groups inoculated with the isolates,with the highest mortality of 80％in one group,indicates that the three Salmonella isolates have different pathoge-nicities.This study provides a reference for the clinical diagnosis and effective prevention of salmonellosis.