犬和猫皮肤癣菌三重荧光定量PCR检测方法的建立及应用OA北大核心CSTPCD

A triplex fluorescent PCR method was established to identify Microsporum canis(MC),Tricho-phyton mentagro phytes(TM)and Malassezia furfur (MF),which was based on the conserved sequences of the ITS genes of the three common dermatopathogenic fungi,namely MC,TM and MF.The primers and probes were designed based on the conserved sequences of the ITS genes of three common dermatopatho-genic fungi,MC,TM and MF,and the triplex fluorescence PCR assay was established.The established trip-lex fluorescence PCR method showed positive amplification only for MC,TM and MF.There was no cross-reactivity with Escherichia coli,Staphylococcus chromogenes and Pseudomonas aeruginosa,which are the common pathogens of canine and feline dermatosis,and the lowest detection limits of MC,TM and MF were 1.36×10 1,1.35×10 1 and 1.33×10 1 copies,respectively.The coefficients of variation of intra-and in-ter-groups were both less than 2％,and the reproducibility was good.The established triplex fluorescent PCR method for MC,TM and MF was applied to 88 clinical samples of dogs and cats,and the results showed that 1 MC,21 TM and 20 MF were detected positively,which was consistent with the single fluo-rescent PCR method.This method can rapidly detect MC,TM and MF at the same time,and can be used for the rapid diagnosis of fungal dermatosis in dogs and cats.