动物医学进展2024，Vol.45Issue(9)：61-66,6.
甘草总黄酮对艰难梭菌的体内外抗菌作用研究
In Vitro and in Vivo Antibacterial Activities of Total Flavonoids from Glycyrrhiza uralensis against Clostridium difficile
摘要
Abstract
The in vitro and in vivo antibacterial activities of total flavonoids from Glycyrrhiza uralensis(TFGU)against Clostridium difficile and the possible mode of action were investigated,which can provide basis for the prevention and treatment of CDI and the efficient utilization of licorice.Micro-broth dilution method and bacterial counting method were applied to determine the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC).The growth curve and killing curve of TFGU on C.difficile were also evaluated.The membrane integrity was investigated by scanning electron microscope,propidium iodide(PI)uptake assay,determination of extracellular ATP and protein.Finally,CDI model was successfully established on mice and in vivo efficacy of TFGU was also evaluated.The results showed that MIC and MBC of TFGU against C.difficile were 12.5 and 25 μg/mL,respectively.TFGU showed a rapid bactericidal manner.Bacterial membrane was significantly disrupted by TFGU,which lead to the leak-age of ATP and protein,morphological change and finally death of bacteria.The body weight decrease of mice was significantly alleviated by TFGU and the 7-day survival rate was 100％,which was equal to van-comycin.In conclusion,TFGU showed significant in vitro and in vivo antibacterial activity against C.dif-ficile and disruption of bacterial membrane maybe the possible mechanism of action.关键词
艰难梭菌/甘草总黄酮/抗菌作用/细胞膜Key words
Clostridium difficile/flavonoids from Glycyrrhiza uralensis/antibacterial activity/bacterial membrane分类
农业科技引用本文复制引用
孙剑峰,任丽蓉,黄博,刘希望..甘草总黄酮对艰难梭菌的体内外抗菌作用研究[J].动物医学进展,2024,45(9):61-66,6.基金项目
甘肃省自然科学基金项目(22JR5RA042),中国农业科学院科技创新工程(25-LZIHPS-02) （22JR5RA042）
