动物医学进展2024，Vol.45Issue(9)：73-76,4.
捻转血矛线虫虫卵孵化及幼虫L3培养条件的优化
Optimization of Hatching Conditions and L3 Culture of Haemonchus contortus
摘要
Abstract
Haemonchosis is a gastrointestinal nematode disease caused by the parasitic infestation of the Haemonchus contortus in ruminant animals like cattle and sheep.This parasite has a wide distribution and significantly impacts the livestock industry.The in vitro cultivation of Haemonchus contortus involves the development of its eggs in a culture medium until they mature into infective third-stage larvae(L3).To op-timize the in vitro culture technique of Haemonchus contortus,a pure and stable strain source is crucial as it lays the foundation for the subsequent experiments of various aspects like antigen and gene isolation and identification,pathogen detection methods,vaccine development,and understanding parasite drug resist-ance.Therefor,This study delves into the effects of various factors on this cultivation process,including the type of culture medium,volume of culture fluid,antibiotics,and the commonly used solvent,dimethyl sul-foxide(DMSO).The NCTC-109 medium emerges as the optimal choice for in vitro cultivation of Haemon-chus contortus.The best cultivation volume is identified as 200ul.The final concentrations of streptomycin and amphotericin B must not exceed 20％and 6.25％,respectively,and the final concentration of DMSO should remain below 0.625％.This indicates that different media,different concentrations of media and the commonly used concentrations of streptomycin,amphotericin B as well as DMSO have different degrees of toxicity on the development of Haemonchus contortus L3.关键词
捻转血矛线虫/体外培养/二甲基亚砜/NCTC-109Key words
Haemonchus contortus/in vitro culture/DMSO/NCTC-109分类
农业科技引用本文复制引用
马梅清,丁玉林,张磊,王瑞,贾晓晴,马园,郝陆瑶,张艳妮,李倩楠,李炎夫,邵瑾,杜山..捻转血矛线虫虫卵孵化及幼虫L3培养条件的优化[J].动物医学进展,2024,45(9):73-76,4.基金项目
国家自然科学基金项目(31960724,32160838,31460656) （31960724,32160838,31460656）
内蒙古自治区重点研发和成果转化计划项目(2023YFDZ0048) （2023YFDZ0048）
内蒙古自然科学基金项目(2023LHMS03022,2023LHMS03005,2021MS03088,2019MS03017) （2023LHMS03022,2023LHMS03005,2021MS03088,2019MS03017）
内蒙古自治区高等学校科学技术研究项目(NJZY21495) （NJZY21495）
