动物医学进展2024，Vol.45Issue(9)：81-87,7.
我国部分省份牛冠状病毒HE基因遗传变异分析
Genetic Variation Analysis of HE Gene of Bovine Coronavirus in Part Areas of China
罗健丹 1张欣怡 2房琳琳 3刘爽 4郑辉 4魏荣 4董雅琴 3肖啸2
作者信息
- 1. 云南农业大学,云南昆明 650500||中国动物卫生与流行病学中心,山东青岛 266032
- 2. 云南农业大学,云南昆明 650500
- 3. 中国动物卫生与流行病学中心,山东青岛 266032||农业农村部动物生物安全风险预警及防控重点实验室(南方),山东青岛 266032
- 4. 中国动物卫生与流行病学中心,山东青岛 266032
- 折叠
摘要
Abstract
In order to understand the prevalence and genetic variation of bovine coronavirus(BCoV)in se-lected provinces of China,a total of 1819 samples including nasal swabs,feces and tissue specimens were collected from 78 clinical cases of affected cattle herds from 14 provinces during the period of 2020-2023.BCoV detection was performed using real-time reverse transcription-polymerase chain reaction(RT-PCR),and a subset of positive samples were subjected to full-length amplification,sequencing,and genetic varia-tion analysis of the HE gene.Among the 78 affected herds,56.41％were tested positive for BCoV.A total of 35 complete HE gene sequences were obtained,with nucleotide homology ranging from 97.0％-100.0％compared to the reference sequences,and amino acid homology ranging from 97.0％-99.8％.Phylogenetic a-nalysis revealed the typical geographical pattern of evolution in BCoV strains,with Asian and American strains showing closer evolutionary relationships,while European strains formed a distinct branch.The se-quenced strains in this study were grouped within the Asia-America clade,closely related to Chinese refer-ence strains.Among the 35 HE sequences,34 showed no insertions or deletions,except for the BCoV/NX13/CHN/2023 strain from Ningxia,which exhibited a 12-nucleotide insertion in the HE gene.This con-firms the presence of BCoV strains with insertional variations in China and suggests the potential for fur-ther dissemination.Genetic recombination analysis indicated a possible recombination event between the BCoV/NX13/CHN/2023 strain and BCoV/HeB120/CHN/2021 strain,with predicted recombination values of 0.797 and 0.759,respectively.This study provides insights into the infection status and genetic variation of BCoV in clinical cases of cattle herds in selected provinces of China,serving as a reference for further characterization of circulating BCoV strains in China.关键词
牛冠状病毒/HE基因/系统进化分析/变异株/基因重组Key words
Bovine coronavirus/HE gene/phylogenetic analysis/variant strain/genetic recombination分类
农业科技引用本文复制引用
罗健丹,张欣怡,房琳琳,刘爽,郑辉,魏荣,董雅琴,肖啸..我国部分省份牛冠状病毒HE基因遗传变异分析[J].动物医学进展,2024,45(9):81-87,7.
