南方医科大学学报2024，Vol.44Issue(8)：1450-1458,9.DOI:10.12122/j.issn.1673-4254.2024.08.03
黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡
Huangqin Qingrechubi Capsule alleviates inflammation and uric acid and lipid metabolism imbalance in rats with gouty arthritis by inhibiting the PTEN/PI3K/AKT signaling pathway
摘要
Abstract
Objective To investigate the effects of Huangqin Qingrechubi Capsule(HQC)on inflammation and uric acid and lipid metabolism in rats with gouty arthritis(GA)and its mechanism.Methods SD rat models of GA established by injecting monosodium urate into the right ankle joint were treated with saline,colchicine and HQC at low,medium and high doses(n=10)by gavage for 7 days.Toe swelling of the rats was detected at 4,8,24,48 and 72 h after modeling,and synovial histological changes were observed with HE staining.Serum levels of interleukin-10(IL-10),IL-18,tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1),adiponectin,leptin,resistin and visfatin were measured by ELISA,and the levels of high-density lipoprotein cholesterol(HDL-C),triglyceride(TG),total cholesterol(TC),and uric acid(BUA)were detected.RT-qPCR and Western blotting were used to detect the mRNA expressions of phosphatase and tensin homolog(PTEN),phosphatidylinositol-3-kinase(PI3K)and protein kinase B(AKT)and the protein expressions of PTEN,PI3K,p-PI3K,AKT and p-AKT.Results The rat models of GA showed obvious toe swelling,which reached the peak level at 48 h.HE staining revealed massive inflammatory cell infiltration and synovial tissue hyperplasia.The rat models showed significantly increased expressions of TNF-α,TGF-β1,IL-18,TC,TG,leptin,resistin and visfatin,BUA,p-PI3K,and p-AKT and lowered levels of IL-10,APN,HDL-C,and PTEN.Treatment with HQC and colchicine obviously improved these changes and alleviated synovial pathologies and toe swelling in the rat models.Conclusion HQC can improve inflammation and correct the imbalance of uric acid and lipid metabolism in GA rats possibly by inhibiting the PTEN/PI3K/AKT signaling pathway.关键词
痛风性关节炎/黄芩清热除痹胶囊/炎症/尿酸代谢/脂质代谢/PTEN/PI3K/AKT信号通路Key words
gouty arthritis/Huangqin Qingrechubi Capsule/inflammation/uric acid metabolism/lipid metabolism/PTEN/PI3K/AKT signaling pathway引用本文复制引用
张先恒,刘健,韩琦,陈一鸣,丁香,陈晓露..黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡[J].南方医科大学学报,2024,44(8):1450-1458,9.基金项目
国家中医药管理局高水平中医药重点学科(ZYYZDXK-2023100) （ZYYZDXK-2023100）
现代中医内科应用基础与开发研究安徽省重点实验室(2016080503B041) （2016080503B041）
安徽省第12批"115"创新团队(皖人才办[2019]1号) （皖人才办[2019]1号）
安徽省名中医刘健工作室建设项目(中医药发展秘[2018]11号) （中医药发展秘[2018]11号）
