南方医科大学学报2024，Vol.44Issue(8)：1497-1507,11.DOI:10.12122/j.issn.1673-4254.2024.08.08
SPAG5在胃癌细胞恶性增殖中的生物学作用
Biological role of SPAG5 in the malignant proliferation of gastric cancer cells
摘要
Abstract
Objective To analyze the expression of SPAG5 in gastric cancer tissues and its regulatory roles in gastric cancer cell growth.Methods TCGA analysis,immunohistochemistry,and immunofluorescence staining were used to analyze the expression patterns of SPAG5 and MKi67 in gastric cancer and adjacent tissues.In gastric cancer AGS and MGC803 cells,the effects of lentivirus-mediated SPAG5 knockdown on cell growth and apoptosis were evaluated using Celigo,MTT,clone formation assays and flow cytometry.Results Proteinatlas and TCGA database analysis suggested that SPAG5 was highly expressed in gastric cancer,and Kaplan-Meier analysis and GEPIA analysis showed high expressions of SPAG 5 in lung adenocarcinoma,breast cancer,hepatocellular carcinoma,pancreatic carcinoma,cervical cancer and bladder carcinoma.Immunohistochemistry revealed that SPAG5 was highly expressed in gastric cancer tissues(P<0.001),and immunofluorescence colocalization analysis demonstrated a significant correlation between SPAG5 and MKI67(R=0.393,P<0.001).RT-qPCR and Western blotting showed that SPAG5 was highly expressed in MKN74,BGC823,MGC803,SGC7901 and AGS cells.In AGS and MGC803 cells,SPAG5 knockdown significantly inhibited proliferation and promoted apoptosis.Conclusions The expressions of SPAG5 and MKi67 are correlated in gastric cancer tissues,and SPAG5 knockdown inhibits the proliferation of gastric cancer cells.SPAG5 is associated with the prognosis of gastric cancer patients and may serve as a promising biomarker for gastric cancer.关键词
SPAG5/胃癌/MKi67/细胞增殖Key words
SPAG5/gastric cancer/MKi67/cell proliferation引用本文复制引用
庞一丹,刘雅,陈思嫒,张荆雷,曾今,潘元明,安娟..SPAG5在胃癌细胞恶性增殖中的生物学作用[J].南方医科大学学报,2024,44(8):1497-1507,11.基金项目
国家自然科学基金(82260846) （82260846）
青海省中藏药管理局科研创新项目(J2022015) （J2022015）
青海省科技厅重点研发与转化计划(2021-QY-213)Supported by National Natural Science Foundation of China(82260846). （2021-QY-213）
