南方医科大学学报2024，Vol.44Issue(8)：1508-1517,10.DOI:10.12122/j.issn.1673-4254.2024.08.09
茵陈蒿汤治疗肝纤维化的核心功能成分群以及潜在通路
Analysis of core functional components in Yinchenhao Decoction and their pathways for treating liver fibrosis
摘要
Abstract
Objective To analyze the core functional component groups(CFCG)in Yinchenhao Decoction(YCHD)and their possible pathways for treating hepatic fibrosis based on network pharmacology.Methods PPI data were extracted from DisGeNET,Genecards,CMGRN and PTHGRN to construct a weighted network using Cytoscape 3.9.1.The data of the chemical components in YCHD were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the potential active components and targets were selected using PreADMET Web server and SwissTargetPrediction.A fusion model was constructed to obtain the functional effect space and evaluate the effective proteins to identify the CFCG followed by GO and KEGG pathway enrichment analyses for all the targets.In cultured human hepatic stellate cells(LX-2 cells),the cytotoxicity of different compounds in YCHD was tested using CCK-8 assay;the effects of these compounds on collagen α1(Col1a1)mRNA expression and the pathways in 20 ng/mL TGF-β1-stimulated cells were analyzed using RT-qPCR and Western blotting.Results A total of 1005 pathogenic genes,226 potential active components and 1529 potential targets in YCHD and 52 potential targets of CFCG were obtained.Benzyl acetate,vanillic acid,clorius,polydatin,lauric acid and ferulic acid were selected for CCK-8 verification,and they all showed minimal cytotoxicity below the concentration of 200 μmol/L.Clorius,polydatin,lauric acid and ferulic acid all effectively inhibited TGF-β1-induced LX-2 cell activation.At the concentration of 200 μmol/L,all these 4 components inhibited PI3K,p-PI3K,AKT,p-AKT,ERK,p-ERK,P38 MAPK and p-P38 MAPK expressions in TGF-β1-induced LX-2 cells.Conclusion The therapeutic effect of YCHD on hepatic fibrosis is probably mediated by its core functional components including benzyl acetate,vanillic acid,clorius,polydatin,lauric acid and ferulic acid,which inhibit the PI3K-AKT and MAPK pathways in hepatic stellate cells.关键词
茵陈蒿汤/核心功能成分群/网络药理学/成分贡献率/肝纤维化/LX-2细胞Key words
Yinchenhao Decoction/core functional component groups/network pharmacology/CDR/hepatic fibrosis/LX-2 cells引用本文复制引用
陈星梅,刘琴文,李镱,钟晓宇,樊奇灵,马柯,罗柳婷,官道刚,朱志博..茵陈蒿汤治疗肝纤维化的核心功能成分群以及潜在通路[J].南方医科大学学报,2024,44(8):1508-1517,10.基金项目
广东省基础与应用基础研究基金(2020A1515010412,2022A1515010121) （2020A1515010412,2022A1515010121）
