南方医科大学学报2024，Vol.44Issue(8)：1599-1604,6.DOI:10.12122/j.issn.1673-4254.2024.08.19
Notch1信号通过调控细胞增殖、迁移、侵袭和糖酵解参与子宫腺肌病的发生及发展
Role of Notch 1 signaling and glycolysis in the pathogenic mechanism of adenomyosis
摘要
Abstract
Objective To investigate the expressions of glycolysis-related factors and changes in Notch1 signaling in endometrial tissues of adenomyosis(AM)and Ishikawa cells to explore the pathogenesis of AM.Methods Eutopic endometrial tissues were collected from 8 patients with AM and 8 patients with uterine fibroids matched for clinical characteristics(control group).The expressions of Notch1 signaling proteins and glycolysis-related factors in the collected tissues were detected using qRT-PCR and Western blotting,and the levels of glucose and lactic acid were determined.An Ishikawa cell model with lentivirus-mediated stable Notch1 overexpression was established for assessing cell survival rate with CCK-8 assay,cell migration and invasion abilities with Transwell migration and invasion assays,and glycolytic capacity by determining the extracellular acidification rate.Results Compared with those in the control group,the endometrial tissues in AM group showed significantly increased expression level of carbohydrate antigen 125(CA125),increased mRNA expression levels of Notch1,HK2 and PDHA and protein expressions of Notch1,GLUT1,HK2,PKM and PDHA,lowered glucose level and increased lactate level.The Ishikawa cell models with stable Notch1 overexpression exhibited significantly increased cell survival rate with attenuated cell migration and invasion abilities and decreased glycolysis capacity and reserve.Conclusion The Notch1 signaling pathway participates in the pathogenesis of AM possibly by regulating the proliferation,migration,invasion and glycolysis of endometrial cells.关键词
子宫腺肌病/子宫内膜/糖酵解/Ishikawa细胞系/慢病毒Key words
adenomyosis/endometrium/glycolysis/Ishikawa/lentivirus引用本文复制引用
温小慧,黄诗雅,刘学红,李坤寅,关永格..Notch1信号通过调控细胞增殖、迁移、侵袭和糖酵解参与子宫腺肌病的发生及发展[J].南方医科大学学报,2024,44(8):1599-1604,6.基金项目
广东省普通高校重点领域专项项目(2023ZDZX2011) （2023ZDZX2011）
广东省普通高校重点领域专项项目(2021ZDZX2015) （2021ZDZX2015）
广州中医药大学"双一流"与高水平大学学科协同创新团队项目 （）
广州中医药大学中医学学科中医基础理论研究"揭榜挂帅"项目 （）
