森林与环境学报2024，Vol.44Issue(5)：484-491,8.DOI:10.13324/j.cnki.jfcf.2024.05.005
锥栗优良无性系遗传多样性的EST-SSR分析
EST-SSR analysis of the genetic diversity of superior clones of Castanea henryi
摘要
Abstract
To promote the selection of new Castanea henryi varieties,the identification of germplasm resources,and the healthy development of the industry,the genetic diversity of 22 excellent clones cultivated in Jian'ou city,Fujian province,were investigated using six pairs of polymorphic EST-SSR primers,and the fingerprints were constructed.Results showed that the average number of alleles per primer pair was 2.667,average number of effective alleles was 2.038,average observed heterozygosity was 0.349,average expected heterozygosity was 0.447,average Shannon's information index was 0.721,average number of polymorphic loci was 11.500,and average polymorphic information content was 0.434,indicating high genetic diversity.The genetic similarity coefficient varied from 0.754 to 1.000,with an average value of 0.856.At the genetic similarity coefficient of 0.860,it could be divided into five major classes.Class Ⅰ included only HL19.Class Ⅱ included HL5,HL6,HL8,and HL21.Class Ⅲ included HL17 and HL18.Class Ⅳ included HL12 and HL13.Class Ⅴ included HL1,HL2,HL3,HL4,HL7,HL9,HL10,HL11,HL14,HL15,HL16,HL20,and HL22.Fingerprints of 22 clones were constructed using three pairs of polymorphic primers.It provided a reference for the selection of parents and superior germplasms.关键词
锥栗/表达序列标签-简单重复序列/无性系/遗传多样性/指纹图谱Key words
Castanea henryi/EST-SSR/clones/genetic diversity/fingerprint mapping分类
农业科技引用本文复制引用
杨金辉,王一帆,李颖林,江锡兵,陈辉,李煜..锥栗优良无性系遗传多样性的EST-SSR分析[J].森林与环境学报,2024,44(5):484-491,8.基金项目
福建农林大学林学高峰学科建设项目(72202200205) （72202200205）
福建农林大学科技创新专项基金项目"锥栗疫病抗性的多群体遗传解析与分子标记辅助选择"(KFB23048). （KFB23048）
