分析化学2024，Vol.52Issue(8)：1072-1081,10.DOI:10.19756/j.issn.0253-3820.241179
基于铁基γ-环糊精纳米粒子的类过氧化物酶活性比色检测肌氨酸
Colorimetric Detection of Sarcosine Based on Peroxidase-like Activity of Fe-based γ-Cyclodextrin Nanoparticles
摘要
Abstract
Fe-based γ-cyclodextrin nanoparticles(Fe-γ-CD)with peroxidase-like activity were successfully synthesized through a solvothermal approach for establishing a simple and sensitive sarcosine(SAR)assay method.Fe-γ-CD could catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine(TMB)to blue oxidized TMB(oxTMB)in the presence of H2O2,accompanied by a characteristic absorption peak centered at 652 nm.The effects of reaction conditions were investigated,and the catalytic mechanism as well as the steady-state kinetics were analyzed.Fe-γ-CD could catalyze H2O2 to generate hydroxyl radical,singlet oxygen and superoxide radical,these reactive oxygen species with robust oxidizability further oxidized TMB.This process followed a typical Michaelis-Menten kinetic model.With the assistance of sarcosine oxidase(SOx),SAR was hydrolyzed into H2O2 to trigger Fe-γ-CD catalyzed chromogenic reaction,resulting in deepened color and increased absorbance.The degree of colorimetric signal change was related to SAR concentration and thus SAR could be quantitatively detected.The linear range and detection limit were 1.0-70.0 μmol/L and 0.46 μmol/L,respectively.Typical amino acids and metal ions had no obvious interference on SAR detection,indicating a good selectivity.The method was successfully applied to determination of SAR level in serum with satisfactory results.关键词
铁基γ-环糊精纳米粒子/肌氨酸/过氧化氢/比色Key words
Fe-based γ-cyclodextrin nanoparticles/Sarcosine/Hydrogen peroxide/Colorimetric引用本文复制引用
王皓,王婷婷,宋浩,刘芳宁,陈传霞,逯一中..基于铁基γ-环糊精纳米粒子的类过氧化物酶活性比色检测肌氨酸[J].分析化学,2024,52(8):1072-1081,10.基金项目
山东省自然科学基金项目(No.ZR2023MB019)、国家自然科学基金项目(No.22172063)和电分析化学国家重点实验室开放课题项目(No.SKLEAC202314)资助. Supported by the Natural Science Foundation of Shandong Province,China(No.ZR2023MB019),the National Natural Science Foundation of China(No.22172063),and the Open Funds of the State Key Laboratory of Electroanalytical Chemistry(No.SKLEAC202314). （No.ZR2023MB019）
