分析化学2024，Vol.52Issue(8)：1082-1093,12.DOI:10.19756/j.issn.0253-3820.241059
纳米酶增强免疫层析法定量检测血液中的降钙素原
Quantitative Detection of Procalcitonin in Blood by Nanozyme-based Lateral Flow Immunoassay
摘要
Abstract
A rapid quantitative immunochromatographic assay for procalcitonin(PCT)using metal-organic frameworks modified with gold and platinum nanoparticles(MAPs)as labels was established in this work.The detection probe was prepared by conjugating MAPs with anti-PCT monoclonal antibody via an electrostatic adsorption method.Anti-PCT polyclonal antibody and sheep anti-mouse IgG were sprayed onto the nitrocellulose(NC)membrane as the test line and quality control line,respectively,to construct immunochromatographic strip for PCT quantitative detection via signal-amplification-based sandwich immunoassay.The results showed that the MAP-based immunochromatographic test had high sensitivity,high specificity,and good stability.The dynamic range for detection of PCT was 0.61 pg/mL-320 ng/mL,the detection limit was 0.25 pg/mL,and the intra-day and inter-day precision(Relative standard deviation)were less than 15%.The results of real sample analysis showed that a quite low volume of sample was required for detection of PCT in whole blood,which was of great significance for the early diagnosis,monitoring and treatment,and prognosis of inflammation.关键词
侧流免疫层析法/降钙素原/快速检测/纳米酶Key words
Lateral flow immunoassay/Procalcitonin/Rapid detection/Nanozyme引用本文复制引用
郑玥,林童,熊勇华,许梦硕,严喜鸾,郭徐静,杨磊,郭亮..纳米酶增强免疫层析法定量检测血液中的降钙素原[J].分析化学,2024,52(8):1082-1093,12.基金项目
江西省自然科学基金项目(No.20232BAB205087)和国家自然科学基金项目(No.82360857)资助. Supported by the Natural Science Foundation of Jiangxi Province(No.20232BAB205087)and the National Natural Science Foundation of China(No.82360857). （No.20232BAB205087）
