分析化学2024，Vol.52Issue(8)：1114-1122,9.DOI:10.19756/j.issn.0253-3820.231180
基于多聚腺嘌呤的二嵌段寡核苷酸探针用于卡那霉素可视化检测
Visual Detection of Kanamycin by Poly-Adenine-based Diblock Oligonucleotide Probe
摘要
Abstract
Adsorption of single-stranded DNA(ssDNA)onto unmodified gold nanoparticles(AuNPs)has been utilized as a common strategy for colorimetric assays,enabling facile detection of target analytes.In this study,a label-free,sensitive AuNP-based colorimetric biosensor was developed for visual detection of kanamycin(Kana)using a diblock oligonucleotide probe(dP)that included a poly-adenine(polyA20)tail.In the presence of Kana,the aptamer on the MBs@double-stranded DNA(dsDNA)complex specifically bound to Kana,leading to dissociation of dPs from complementary aptamer strands.The dP sequences were anchored to the surface of AuNPs due to the strong affinity between polyA20 tail and AuNPs,thus stabilizing the colloidal AuNPs against aggregation when high concentration level salt(NaCl)was introduced.Compared with random sequence(RS)with the same number of bases,AuNPs colorimetric strategy with polyA20 tail sequence significantly improved the detection sensitivity of Kana by two orders of magnitude.The results showed that the colorimetric sensor had good colorimetric performance when the concentration of Kana ranged from 0.01 to 1 nmol/L,and the detection limit(S/N=3)was 0.01 nmol/L.This sensing system did not require large instruments and other signal amplification technique,and was suitable for visualization and real-time detection of large quantities of samples with high sensitivity and specificity.This study provided a good idea for visual detection of other antibiotic drugs and chemical pollutants affecting food safety.关键词
卡那霉素/金纳米颗粒/核酸适配体/生物传感器/食品安全Key words
Kanamycin/Gold nanoparticles/Aptamer/Biosensor/Food safety引用本文复制引用
席玥,王欣雨,王汇元,颜娟..基于多聚腺嘌呤的二嵌段寡核苷酸探针用于卡那霉素可视化检测[J].分析化学,2024,52(8):1114-1122,9.基金项目
上海市自然科学基金(No.20ZR1424100)和国家市场监管重点实验室(生物分析计量溯源领域)开放课题(No.KLSMR2022-01)资助. Supported by the Natural Science Foundation of Shanghai(No.20ZR1424100)and the Key Laboratory of State Administration of Market Regulation of China(No.KLSMR2022-01). （No.20ZR1424100）
