分析化学2024，Vol.52Issue(8)：1123-1131,9.DOI:10.19756/j.issn.0253-3820.210896
基于新型发夹核酸探针和G-四链体/血红素脱氧核酶的多功能生物传感器
A New Multifunctional Biosensor Based on a Hairpin Oligonucleotide Probe and G-Quadruplex/Hemin DNAzyme
摘要
Abstract
A new multifunctional fluorescent"turn-on"biosensor was constructed based on a guanine-rich hairpin probe and G-quadruplex/hemin DNAzyme.The probe was labeled with quencher and fluorescein at 5′and 3′terminus,respectively.In the absence of the target,the probe form hairpin structure and the fluorescence of the fluorescein was quenched by the quencher.However,in the presence of the target,the hairpin structure transformed to G-quadruplex and the fluorescence was recovered.By monitoring the fluorescence of the probe before and after combining with the target,both hemin and hemoglobin were detected rapidly and conveniently with high sensitivity.The limit of detection for hemin and hemoglobin were estimated to be 283 pmol/L and 109 pmol/L,respectively.The specific interaction between the DNA probe and hemin were investigated via absorption and fluorescence spectra.Furthermore,L-cysteine was detected with naked eyes based on the peroxidase-like activity of the new G-quadruplex/hemin DNAzyme.The limit of detection for L-cysteine was estimated to be 3.6 μmol/L,which was lower than the normal level of intracellular L-cysteine(30-200 μmol/L).The method developed here was rapid,simple and sensitive for detection of hemin,hemoglobin and L-cysteine,demonstrating great potential in bioanalysis,drug screening,and disease diagnosis.关键词
G-四链体/脱氧核酶/血红素/血红蛋白/生物传感器Key words
G-Quadruplex/DNAzyme/Hemin/Hemoglobin/Biosensor引用本文复制引用
王蕾,宋吉祥,刘兴奋,黄艳琴,范曲立..基于新型发夹核酸探针和G-四链体/血红素脱氧核酶的多功能生物传感器[J].分析化学,2024,52(8):1123-1131,9.基金项目
江苏高校优势学科建设工程项目(No.YX030003)和南京邮电大学科研项目(Nos.NY222151,NY221091)资助 Supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.YX030003)and the Program of Nanjing University of Posts and Telecommunications(Nos.NY222151,NY221091). （No.YX030003）
