分析化学2024，Vol.52Issue(8)：1132-1140,中插6-中插11,15.DOI:10.19756/j.issn.0253-3820.241014
卟啉-吡啶基共轭寡聚物荧光纳米探针用于次氯酸的灵敏检测
Sensitive Detection of Hypochlorous Acid Using Porphyrin-pyridyl Conjugated Oligomer-based Fluorescent Nanoprobe
摘要
Abstract
Conjugated oligomers,as analogues of conjugated polymers,allow for constructing ultrafine fluorescent nanoparticles with improved sensing sensitivity due to their more precisely defined molecular structure and smaller size.Herein,a new porphyrin-pyridyl conjugated oligomer,i.e.,Zn-TPP-OMe,was designed and synthesized via Sonogashira coupling strategy and a super-small(～2.0 nm)water-soluble fluorescent nanoprobe(Zn-TPP-OMe nanoparticles(NPs))was subsequent obtained by nanoprecipitation method.Due to the addition reaction of chlorine atom to porphyrin ring,the fluorescence intensity of Zn-TPP-OMe NPs at 619-660 nm significantly decreased with increasing HCLO concentration.Meanwhile,Zn-TPP-OMe NPs aggregated obviously after adding HClO,achieving enhanced fluorescence quenching.The fluorescence detection mechanism was verified by transmission electron microscopy(TEM),proton nuclear magnetic resonance(1H NMR)and high resolution mass spectrometry(HRMS)analyses.The experimental results indicated that the porphyrin-pyridyl conjugated oligomer nanosystem had high sensitivity(LOD=0.005 μmol/L)and excellent selectivity in detection of HClO,and was successfully applied to the sensing of HClO in actual water samples.关键词
共轭寡聚物/反应性荧光探针/次氯酸/卟啉/荧光传感Key words
Conjugated oligomer/Reactive fluorescence probe/Hypochlorous acid/Porphyrin/Fluorescence sensing引用本文复制引用
黄际艳,陈米雪,李金玲,肖毅,黄红梅,张友玉..卟啉-吡啶基共轭寡聚物荧光纳米探针用于次氯酸的灵敏检测[J].分析化学,2024,52(8):1132-1140,中插6-中插11,15.基金项目
国家自然科学基金(No.21475042)、湖南省自然科学基金(No.2023JJ30389)和湖南省教育厅科学研究基金项目(No.22C0030)资助. Supported by the National Natural Science Foundation of China(No.21475042),the Natural Science Foundation of Hunan Province,China(No.2023JJ30389)and the Project Supported by the Research Foundation of Education Department of Hunan Province,China(No.22C0030). （No.21475042）
