广东医学2024，Vol.45Issue(8)：959-969,11.DOI:10.13820/j.cnki.gdyx.20235054
MiR-139-5p通过ULK1减弱细胞自噬而抑制非小细胞肺癌
MiR-139-5p suppresses autophagy and inhibits non-small cell lung cancer by targeting ULK1
摘要
Abstract
Objective To evaluate the impact of microRNAs(miRNAs)on lung cancer and elucidate the specif-ic mechanism,providing theoretical basis for the development of anti-cancer biological agents.Methods Differential expression of miRNAs in lung cancer tissues and normal lung tissues was identified by sequencing.The relationship be-tween miRNAs and lung cancer was validated using data from The Cancer Genome Atlas(TCGA)database.Cell viability,invasion,migration,and anti-apoptotic capacity were assessed using Cell Counting Kit-8(CCK-8),transwell,and flow cytometry assays after miRNA intervention.Bioinformatics analysis of miRNA target genes and functional validation were conducted.Results Sequencing revealed decreased expression of miR-139-5p in lung cancer tissues compared to normal lung tissues.TCGA data confirmed the role of miR-139-5p in lung cancer,suggesting its potential as a tumor suppressor.Functional assays demonstrated that miR-139-5p inhibited the viability,invasion,and anti-apoptotic ca-pacity of lung cancer cells.Mechanistic studies revealed the involvement of autophagy in the development of lung cancer,and miR-139-5p directly targeted and inhibited the autophagy gene unc-51-like kinase 1(ULK1),thereby sup-pressing autophagy in lung cancer.Downregulation of ULK1 reduced the viability,invasion,and anti-apoptotic capacity of lung cancer cells,while upregulation of ULK1 had the opposite effects.Conclusion MiR-139-5p attenuates auto-phagy in non-small cell lung cancer by targeting ULK1,thereby weakening the carcinogenic effects of autophagy.关键词
非小细胞肺癌/自噬/ULK1/miR-139-5pKey words
non-small cell lung cancer/autophagy/ULK1/miR-139-5p分类
医药卫生引用本文复制引用
江泽勇,赵健,吴文杰,陈国硕..MiR-139-5p通过ULK1减弱细胞自噬而抑制非小细胞肺癌[J].广东医学,2024,45(8):959-969,11.基金项目
广东省基础与应用基础研究基金(2019A1515010026) （2019A1515010026）
广州市卫生健康科技项目(20221A010058) （20221A010058）
