寒旱农业科学2024，Vol.3Issue(8)：711-723,封3,14.DOI:10.3969/j.issn.2097-2172.2024.08.005
基于高温高湿条件下苹果加工过程中代谢产物的多样性分析
Analysis of Metabolite Diversity in Apple Processing Based on High Temperature and High Humidity Conditions
摘要
Abstract
By analyzing the intrinsic mechanisms of how fermentation affects apple quality,this study provides a scientific basis for the functional components and metabolic regulation mechanisms of apples,supporting the in-depth development of the apple industry.In this research,non-targeted metabolomics technology was utilized to analyze the diverse differential metabolic compounds generated during the processing of apples under high temperature and high humidity conditions.Various methods such as principal component analysis(PCA),cluster heat maps,partial least squares discriminant analysis(PLS-DA),and KEGG pathway enrichment were utilized to evaluate the metabolomic data.PCA revealed significant stage-wise changes in the metabolic composition of apples during different fermentation processes.Metabolite identification indicated the presence of 314 metabolites,with lipids and lipid-like molecules accounting for the largest proportion,reaching 44.61%.Utilizing cluster dendrograms and orthogonal partial least squares discriminant analysis(OPLS-DA),it was revealed that the metabolite levels in the apple samples at the end of fermentation were significantly higher than those in the unfermented samples,effectively distinguishing apple samples at different fermentation stages.Further,KEGG pathway analysis pointed out that the biosynthesis of phenylpropanoids and the metabolism of phenylalanine in apples were very active during fermentation,and specific pathways for the biosynthesis of cornoside and sphingolipid metabolism were activated under high temperature conditions,which have an important impact on the high-temperature adaptability and flavor formation of apples.关键词
非靶向代谢组学/苹果/高温发酵/代谢途径/代谢产物/多样性/质量控制/风味形成Key words
Non-targeted metabolomics/Apple/High-temperature fermentation/Metabolic pathway/Metabolic product/Di-versity/Quality control/Flavor formation分类
农业科技引用本文复制引用
宋娟,康三江,张海燕,曾朝珍,袁晶,慕钰文,苟丽娜..基于高温高湿条件下苹果加工过程中代谢产物的多样性分析[J].寒旱农业科学,2024,3(8):711-723,封3,14.基金项目
甘肃省农业农村厅科技项目(GNKJ-2022-29) （GNKJ-2022-29）
国家苹果产业技术体系(CARS-27-2024) （CARS-27-2024）
甘肃省科技计划项目(23JRRA1792). （23JRRA1792）
