广西植物2024，Vol.44Issue(8)：1458-1468,11.DOI:10.11931/guihaia.gxzw202401056
海草床盐生植物根际土壤可培养细菌多样性及抑菌活性研究
Diversity and antibacterial activity of culturable bacteria from rhizosphere soil in seagrass bed halophytes
摘要
Abstract
The purpose of this study was to investigate the novelty and particularly of the culturable bacteria in seagrass bed and study its antibacterial activity crude extracts of the metabolites.A total of nine rhizosphere soil samples were collected from the seagrass bed ecosystems in Pearl Bay and Yuzhouping,Fangchenggang City.Five different isolation media were used to isolate and purify the culturable bacteria using the dilution spread plate method.The bacterial species were identified through PCR amplification and 16S rRNA sequencing.The antibacterial activity against four human pathogenic bacteria(methicillin-resistant Staphylococcus aureus,S.epidermidis,S.aureus and Acinetobacter baumannii)were screened with Kirby-Bauer method.The results were as follows:(1)A total of 42 culturable bacteria were isolated,belonging to 14 genera and 13 families,which included Streptomyces,Vibrio,Bacillus,Shewanella,Microbacterium,Brevibacterium,Staphylococcus,Sphingomonas,Rothia,Psychrobacter,Pseudonocardia,Photobacterium,Halomonas,and Shigella.The genus Streptomyces was the dominant bacterium.(2)Antibacterial activity showed that six bacteria exhibited strong inhibitory activity against at least two or more human pathogenic bacteria,which included Vibrio(1 strain),Rothia(1 strain),and Streptomyces(4 strains).The studies show that the rhizosphere soil microbial resources of halophytes in the seagrass bed in Fangchenggang sea area are novel and unique,and Streptomyces has a strong antibacterial activity,which provides a new source for the research and development of new antibiotics drugs.关键词
海草床/人类致病菌/细菌多样性/链霉菌属/抑菌活性Key words
seagrass bed/human pathogenic bacteria/bacteria diversity/Streptomyces/antibacterial activity分类
农业科技引用本文复制引用
陈望安,李蜜,黎芳婷,高程海..海草床盐生植物根际土壤可培养细菌多样性及抑菌活性研究[J].广西植物,2024,44(8):1458-1468,11.基金项目
国家自然科学基金(81903533) （81903533）
广西重点研发计划项目(桂科AB24010109) （桂科AB24010109）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
广西中医药大学桂派杏林拔尖人才资助项目(2022C008) （2022C008）
广西中医药大学高层次人才培育创新团队项目(2022A007). （2022A007）
