工业微生物2024，Vol.54Issue(4)：43-47,5.DOI:10.3969/j.issn.1001-6678.2024.04.015
超声辅助低共熔溶剂葛根多糖提取工艺及其抗氧化活性研究
Ultrasonic-assisted Deep Eutectic Solvent Extraction Process and Antioxidant Activity of Pueraria Lobata Polysaccharides
摘要
Abstract
This paper researched the optimal extraction process of Pueraria lobata polysaccharides by using ultrasonic-assisted deep eutectic solvent and the antioxidant activity of Pueraria lobata polysaccharides.Taking the Pueraria as the raw material and the ultrasonic-assisted deep eutectic solvent as the extraction method,the extraction process of Pueraria lobata polysaccharides was optimized by single factor and Box-Behnken method,and the antioxidant activity of Pueraria lobata polysaccharides was studied by DPPH method.Through the optimization of deep eutectic solvents,choline chloride-ethylene glycol 1∶2 was determined as the optimal extraction solution,detailed as follows:extraction time 35 min,extraction temperature 40 ℃,liquid-material ratio 14 mUg,and the average extraction rate of Pueraria lobata polysaccharides was 13.27％.When the concentration of Pueraria lobata polysaccharides was 1.00 mg/mL,the highest scavenging rate of DPPH free radicals was up to 88.72％.The ultrasonic-assisted deep eutectic solvent extraction of Pueraria lobata polysaccharides had a high extraction rate,was environmentally friendly,and could preserve the activity of the target product.关键词
葛根多糖/低共熔溶剂/响应面法/抗氧化活性Key words
Pueraria lobata polysaccharides/deep eutectic solvents/response surface methodology/antioxidant activity引用本文复制引用
廉伟伟,吕双双,王春燕,刘金海,郝培娜,张会丽..超声辅助低共熔溶剂葛根多糖提取工艺及其抗氧化活性研究[J].工业微生物,2024,54(4):43-47,5.基金项目
河南省高等教育学会高等教育研究重点项目(2021SXHLX029) （2021SXHLX029）
郑州工业应用技术学院校级科研项目(2023YB011). （2023YB011）
