工业微生物2024，Vol.54Issue(4)：167-169,3.DOI:10.3969/j.issn.1001-6678.2024.04.047
过敏性猝死大鼠血清IgE、MCT及咽喉和胃壁组织内P物质的检测及意义
Detection and Significance of IgE and MCT in the Serum,and Substance P in the Throat and Gastrontestinal Tissues of Rats with Sudden Allergic Death
李龙美 1张兰兰 1窦贵1
作者信息
- 1. 贵州名鉴司法鉴定中心,贵州贵阳 550081
- 折叠
摘要
Abstract
This paper aims to explore the detection and significance of IgE,mast cell trypsin(MCT)in serum,and substance P(SP)in the throat and gastrointestinal tissues of rats with sudden allergic death.Fourteen healthy and clean SD rats were selected and divided randomly into a sensitization group and a 0.9％soduim chloride solution control group,with seven rats in each group.Enzyme linked immunosorbent assay(ELISA)was used to detect IgE and MCT levels in rat serum,while immunohistoch-emistry was used to detect the SP levels in throat and gastrointestinal tissues.The results showed that IgE and MCT levels in the serum of rats with sudden allergic death increased,with the significant difference compared with the normal group(P＜0.05).The differences of SP levels in the throat and gastrointestinal tissues between the experimental group and the normal group was statistically significant(P＜0.05).The IgE and MCT in the setum,and the SP in the throat and gastrointestinal tissues can be used as forensic pathological diagnostic indicators for allergic shock in rats.关键词
过敏/猝死/IgE/MCT/咽喉和胃壁组织/P物质Key words
allergy/sudden death/IgE/MCT/throat and gastrointestinal tissues/substance P引用本文复制引用
李龙美,张兰兰,窦贵..过敏性猝死大鼠血清IgE、MCT及咽喉和胃壁组织内P物质的检测及意义[J].工业微生物,2024,54(4):167-169,3.
