玉米DUF668基因家族全基因组鉴定及其表达分析OA北大核心CSTPCD

The domain of unknown function 668(DUF668)family is a family of plants whose function is unknown.To unveil the function and characteristics of maize DUF668(ZmDUF668)gene family,we identified ZmDUF668 gene family by bioinformatics method.The results showed that there were 19 ZmDUF668 genes in maize,distributing on 8 chromo-somes,named ZmDUF668-1-ZmDUF668-19;the most of proteins encoded by ZmDUF668 were alkaline,and most of the family members were localized in the nucleus,cytoplasm and chloroplast.ZmDUF668 family proteins can be divided into two subfamilies according to the multispecies phylogenetic tree.Ten Motifs were identified from 19 members of Zm-DUF668,all of which contained Motif 1 and Motif 5.Through the analysis of protein conserved domain,it was found that 14 of the 19 members contained not only DUF668 domain but also DUF3475 domain.Gene structure analysis showed that members of the same subfamilies had similar gene structure.Synteny analysis showed that there were 21 collinear rela-tionships between 13 ZmDUF668 genes and 10 OsDUF668 genes.The analysis of cis-acting elements revealed that the promoters of ZmDUF668 genes widely contained cis-elements that light-response,plant hormones and abiotic stress.The prediction results of protein-protein interaction network(PPI)indicated that only ZmDUF668-10 of the ZmDUF668 family proteins had interaction with other proteins.Analysis of RNA-Seq revealed that the gene expression level of some Zm-DUF668 gene family members changed significantly under cold,heat,salt stress and ultraviolet treatment.The response of ZmDUF668 family genes to cold and heat stress was verified by RT-qPCR.Bioinformatics was applied to the analysis of the ZmDUF668 gene family,unveiling the characteristics of the members of the ZmDUF668 gene family,and providing theoretical basis for the subsequent study of the molecular biological function of the ZmDUF668 gene family.