小麦籽粒钙和钾含量的QTL定位及遗传效应分析OA北大核心CSTPCD

Calcium and potassium are important mineral nutrient elements in wheat.It is significant to explore the related genetic mechanisms and effects on human nutritional health.To provide a theoretical basis for biofortifi-cation breeding of trace elements in wheat grains,we used 164 F6 recombinant inbred lines(RILs)derived from Avo-cet/Chilero(AC)and 175 F6 RILs derived from Avocet/Huites(AH).Our investigation focused on phenotypic vari-ations in grain calcium(GCa)and grain potassium(GK)content in five environments.QTL mapping was conducted with diversity arrays technology(DArT)chip.Nineteen QTLs associated with grain calcium content were identi-fied,distributed on chromosomes 1A,1D,2A,2B,3 A,3D,4A,4B,4D,5A,5B,7A,7B,and 7D,explaining 3.23％-16.29％of phenotypic variation.Simultaneously,23 QTLs linked to grain potassium content were identified on chromosomes 1B,2A,2B,3A,3B,4A,4D,5A,6A,6B,and 7D,explaining 3.31％-24.66％of phenotypic variation.QGCa.haust-1A,QGCa.haust-AC-5A and QGK.haust-AC-2A.2 were located in multiple en-vironments.QGCa.haust-1A and QGCa.haust-AC-5A explained 7.82％-12.72％and 9.68％-15.57％of phenotypic variation,and the physical intervals were 498.67-532.21 Mb and 461.52-486.26 Mb,respective-ly.QGK.haust-AC-2A.2 explained 8.15％-15.20％of phenotypic variation,with a physical range of 354.61-462.37 Mb.The genetic effect analysis of QGCa.haust-1A,QGCa.haust-AC-5A,and QGK.haust-AC-2A.2 showed that each locus effectively increased the calcium and potassium content in wheat grain.Aggregation effect analysis indicated that the lines with QGCa.haust-1A and QGCa.haust-AC-5A effect loci had highly significantly higher calcium content than those with only a single locus.In summary,three stable loci of grain calcium and po-tassium content are mapped on chromosomes 1 A,2 A,and 5 A,which could significantly increase calcium and po-tassium content in wheat grain.