华北农学报2024，Vol.39Issue(4)：47-53,7.DOI:10.7668/hbnxb.20194546
甘蓝型油菜转录因子BnHY5-2参与盐碱胁迫的响应
Response of the Transcription Factor BnHY5-2 in Brassica napus L.to Salt and Alkali Stress
摘要
Abstract
The transcription factor BnHY5-2 is associated with plant stress resistance.In order to reveal the re-sponse of Brassica napus L.transcription factor BnHY5-2 to salt alkali stress in B.napus,the response of BnHY5-2 to light and salt and alkali was analyzed by transient overexpression,qRT-PCR analysis and subcellular localization.The results revealed that under light conditions,the expression level of the BnHY5-2 gene in B.napus leaves and stems was 29.22 and 3.15 fold higher,respectively,compared to dark conditions.The higher sensitivity to light in leaves suggested that they were the primary site for light signal response.Under light conditions,the expression of BnHY5-2 in leaves and stems was significantly downregulated by 53.1％and 31.0％,respectively,when B.napus was planted in Dalian coastal saline-alkali soil;after applying saline-alkali treatment under dark conditions,the ex-pression of BnHY5-2 was downregulated by 48.2％in the stem,while the difference in expression in the leaves was not significant,indicating organ differences,indicating that the leaves had stricter requirements for light conditions.In B.napus leaves with transient overexpression of BnHY5-2,two out of six genes related to saline-alkali stress(BnNAC32 and BnGS)showed upregulation by 1.25,3.28 fold,respectively,while the other four genes(Bnamy,BnAsp,BnNHX7,BnTPS)were downregulated by 24.8％,25.4％,71.0％,and 82.0％,respectively.Meanwhile,the content of the resistance substance betaine in B.napus increased from 0.256 to 0.573 mg/g,indicating an enhancement by 1.24 fold,suggesting that the overexpression of BnHY5-2 gene could improve the saline-alkali tolerance of B.napus.Sub-cellular localization results showed that the transcription factor BnHY5-2 was localized in the nucleus and regulates the expression of functional genes.Therefore,BnHY5-2 is not only related to light signaling but also participates in the saline-alkali resistance of Brassica napus L.关键词
甘蓝型油菜/BnHY5-2/盐碱胁迫/过表达/转录因子Key words
Brassica napus L./BnHY5-2/Saline-alkali stress/Overexpression/Transcription factor分类
农业科技引用本文复制引用
杨明煊,李明玉,汪波,王泽,刘智强,周广生,于放,刘志文..甘蓝型油菜转录因子BnHY5-2参与盐碱胁迫的响应[J].华北农学报,2024,39(4):47-53,7.基金项目
作物遗传改良国家重点实验室开放基金(20200902) （20200902）
辽宁省教育厅科学研究项目(LJKZ0523) （LJKZ0523）
大连工业大学国家级大学生创新创业项目(202310152015) （202310152015）
