河北医学2024，Vol.30Issue(8)：1255-1261,7.DOI:10.3969/j.issn.1006-6233.2024.08.05
健脾化痰祛瘀法对肥胖2型糖尿病模型糖脂代谢及IRS-1/PI3K/Akt2信号通路的影响
Stasis-Removing Method on Glucose and Lipid Metabolism and the IRS-1/PI3K/Akt2 Signaling Pathway in an Obese Type 2 Diabetes Mellitus Model
摘要
Abstract
Objective:To explore the effects and mechanisms of the spleen-fortifying,phlegm-resol-ving,and stasis-removing method on glucose and lipid metabolism in rats with type 2 diabetes mellitus(T2DM).Methods:T2DM was induced in rats using a high-fat diet combined with a low dose of streptozoto-cin.The rats were divided into control,model,and three treatment groups with low,medium,and high doses of the spleen-fortifying,phlegm-resolving,and stasis-removing formula.Serum inflammatory cytokines,bio-chemical indicators,and key enzyme activities related to glucose metabolism in the liver were measured using ELISA kits.mRNA levels of key insulin signaling pathway targets were detected by qPCR.Western blotting was used to assess the expression of phosphatidylinositol 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),phosphorylated Akt(p-Akt),and glucose transporter 2(Glut2)in the liver.Re-sults:Compared with the control group,the model group had significantly elevated serum levels of fasting blood glucose(FBG),fasting insulin(FINS),total cholesterol(TC),triglycerides(TG),free fatty acids(FFA),and low-density lipoprotein cholesterol(LDL-C),and significantly reduced high-density lipopro-tein cholesterol(HDL-C).The treatment with the spleen-fortifying,phlegm-resolving,and stasis-removing formula significantly reduced FINS,TG,TC,LDL-C,and FFA levels,while increasing HDL-C levels com-pared with the model group.Additionally,serum levels of CRP,IFN-γ,TNF-α,IL-6,IL-1β,and NO were significantly higher in the model group compared to the control group,but were significantly reduced after treatment.The activities of PEPCK,FBPase,G6Pase,and GP enzymes were elevated in the model group but decreased significantly following treatment.Moreover,the expression of IRS-1,p-PI3K,and p-Akt2 was in-creased in the model group but significantly reduced with the treatment.Conclusion:The spleen-fortifying,phlegm-resolving,and stasis-removing method may improve glucose and lipid metabolism in T2DM rats by activating the IRS-1/PI3K/Akt2 signaling pathway.关键词
2型糖尿病/健脾化痰祛瘀法/糖脂代谢/IRS-1/PI3K/Akt2信号通路Key words
Type 2 diabetes mellitus/Spleen-fortifying phlegm-resolving and stasis-removing method/Glucose and lipid metabolism/IRS-1/PI3K/Akt2 signaling pathway引用本文复制引用
敦志华,仇亚茹,魏秀风,梅建强,张明,成立娟,武海淼..健脾化痰祛瘀法对肥胖2型糖尿病模型糖脂代谢及IRS-1/PI3K/Akt2信号通路的影响[J].河北医学,2024,30(8):1255-1261,7.基金项目
河北省中医药管理局2022年度中医药类科研计划课题,(编号:2022405) （编号:2022405）
