柳叶蜡梅PAL基因家族的鉴定及表达分析OA北大核心CSTPCD

Phenylalanine ammonia-lyase(PAL)is a critical rate-limiting enzyme in phenylpropane metabolism,which plays an essential role in plant growth and stress response.To investigate the role of the PAL gene family in the growth and development of Chimonanthus salicifolius,physicochemical properties,cis-acting elements,conserved motifs,phylogenetic tree,chromosomal localization,and collinearity relationship were systematically investigated in this study.The expression patterns in different tissues,at various growth stages of leaves and flowers,and under the treatment of abscisic acid(ABA)and methyl jasmonate(MeJA)were compared.The results showed that 7 PAL family genes were located on 5 chromosomes,encoding proteins with 413-768 amino acids.They were classified into 2 subfamilies,showing close relationships with CpPAL proteins,and exhibited gene tandem duplications.A large number of cis-acting elements containing hormone responses and stress responses were enriched in the promoter regions of CsPAL family genes.The CsPAL family genes were differentially expressed in different tissues,at different developmental stages of leaves and flowers,and under the treatment of ABA and MeJA.CsPAL6 was highly expressed in leaves,and induced by ABA and MeJA,which was associated with the reduction of flavonoids,suggesting that CsPAL6 might be involved in the biosynthesis of flavonoids.The results provide a theoretical reference for further study the role of CsPAL gene family genes in the biosynthesis of C.salicifolius flavonoids.