核农学报2024，Vol.38Issue(10)：1836-1846,11.DOI:10.11869/j.issn.1000-8551.2024.10.1836
柳叶蜡梅PAL基因家族的鉴定及表达分析
Identification and Expression Patterns of PAL Gene Family in Chimonanthus salicifolius
摘要
Abstract
Phenylalanine ammonia-lyase(PAL)is a critical rate-limiting enzyme in phenylpropane metabolism,which plays an essential role in plant growth and stress response.To investigate the role of the PAL gene family in the growth and development of Chimonanthus salicifolius,physicochemical properties,cis-acting elements,conserved motifs,phylogenetic tree,chromosomal localization,and collinearity relationship were systematically investigated in this study.The expression patterns in different tissues,at various growth stages of leaves and flowers,and under the treatment of abscisic acid(ABA)and methyl jasmonate(MeJA)were compared.The results showed that 7 PAL family genes were located on 5 chromosomes,encoding proteins with 413-768 amino acids.They were classified into 2 subfamilies,showing close relationships with CpPAL proteins,and exhibited gene tandem duplications.A large number of cis-acting elements containing hormone responses and stress responses were enriched in the promoter regions of CsPAL family genes.The CsPAL family genes were differentially expressed in different tissues,at different developmental stages of leaves and flowers,and under the treatment of ABA and MeJA.CsPAL6 was highly expressed in leaves,and induced by ABA and MeJA,which was associated with the reduction of flavonoids,suggesting that CsPAL6 might be involved in the biosynthesis of flavonoids.The results provide a theoretical reference for further study the role of CsPAL gene family genes in the biosynthesis of C.salicifolius flavonoids.关键词
柳叶蜡梅/PAL/表达分析/黄酮/脱落酸Key words
Chimonanthus salicifolius/PAL/expression analysis/flavonoid/abscisic acid引用本文复制引用
张桂华,李贺鹏,岳春雷,俞振明..柳叶蜡梅PAL基因家族的鉴定及表达分析[J].核农学报,2024,38(10):1836-1846,11.基金项目
浙江省省属科研院所扶持专项(2024F1068-2),国家自然科学基金项目(32000257),浙江省基础公益研究计划项目(LY23H280003),浙江中医药大学教育教学改革项目(YB23023) （2024F1068-2）
