魔芋海藻糖-6-磷酸合酶基因家族鉴定及表达分析OA北大核心CSTPCD

Trehalose-6-phosphate Synthase(TPSs)play an important role in plant growth and stress tolerance.To investigate the role of the AkTPSs family in the growth and secondary metabolism of Amorphophallus konjac(A.konjac),the physicochemical properties,phylogenetic relationships,gene structure,chromosomal localization,and protein collinearity of the AkTPSs family were analyzed.Additionally,the expression levels of AkTPSs in various tissues and during corm growth stages of A.konjac were investigated.Quantitative real-time PCR(qRT-PCR)was utilized to assess the expression of selected AkTPSs targets following lanthanum induction.The results revealed that 15 AkTPSs genes were randomly distributed in 5 chromosomes.The proteins encoded by these genes vary in length from 278 to 867 amino acids,with all the AkTPSs being hydrophilic.The AkTPSs proteins were divided into 2 subfamilies with distinct functions by phylogenetic analysis,showing close relationships with OsTPSs proteins.AkTPSs have gene tandem duplications.Promoter cis-acting element analysis indicated that AkTPSs contain several cis-elements related to plant hormones,growth,and other functions.Transcriptome data combined with lanthanum-induced qRT-PCR analysis revealed that AkTPS14 played significant roles in regulating both the growth and the accumulation of glucan in the A.konjac corm.These results provide a theoretical reference for further studies on AkTPSs genes and their biological functions in A.konjac.