谷子SiNADP-ME1基因的光响应模式OA北大核心CSTPCD

In order to investigate the response patterns of the photosynthetic gene SiNADP-ME1(Seita.3G109300)to different light intensity and light quality in foxtail millet(Setaria italica),reverse transcription polymerase chain reaction(RT-PCR)was utilized to clone the CDS sequence of SiNADP-ME1.The MEGA(Molecular Evolutionary Genetics Analysis)software was used to construct multi-species evolutionary tree.Additionally,the PCAMBIA1300-SiNADP-ME1-GFP fusion expression vector was constructed and transformed into tobacco(Nicotiana tabacum)leaf cells by Agrobacterium tumefaciens-mediated transformation for subcellular localization.Quantitative real-time PCR(qRT-PCR)was used to detect the expression levels of SiNADP-ME1 genes under different light intensity and light quality.The results revealed that the CDS length of SiNADP-ME1 was 1731 bp,encoding 576 amino acids.Multiple sequence alignment and phylogenetic tree analysis indicated that SiNADP-ME1 was closely related to SvNADP-ME1(XP_03458422.1)of Setaria viridis.Subcellular localization showed that SiNADP-ME1 was localized in the endoplasmic reticulum.The expression pattern of SiNADP-ME1 varied significantly in different tissues at different growth stages of foxtail millet,with the highest expression level in panicle at the grain filling stage.Furthermore,it was found that SiNADP-ME1 responded to different light intensity and light quality.The expression level of SiNADP-ME1 reached its maximum at 3 h under 1200 µmol·m-2·s-1,which was 4.25 times higher than the control(under 250 µmol·m-2·s-1 light intensity).Red light had a dominant effect on the expression of SiNADP-ME1,which was 47.27 times higher than that under dark and 2.69 times higher than that under white light.This study provides some references for further study on the biological function of the SiNADP-ME1 gene.