核农学报2024，Vol.38Issue(10)：1922-1929,8.DOI:10.11869/j.issn.1000-8551.2024.10.1922
多维度评价电子束辐照灭菌对白花蛇舌草质量的影响
Multidimensional Evaluation on the Effect of Electron Beam Irradiation Sterilization on the Quality of Herba Hedyoti Diffusae
摘要
Abstract
To evaluate the application of electron beam irradiation sterilization technology in Herba Hedyoti Diffusae from multiple dimensions,its effect on the microbial level,chemical composition content,fingerprint,antioxidant activity,and cell survival rate of the sample materials was investigated.The results showed that irradiation could greatly sterilize the microorganisms in Herba Hedyoti Diffusae.The content of rutin and luteolin,as well as the three antioxidant activities and cell survival rate were not significantly different from these before sterilization(P>0.05)at an irradiation dose of 2-10 kGy.When the irradiation dose was greater than 10 kGy,the antioxidant activity in vitro decreased,and the content of hexadecane methyl ester decreased.It is recommended to use a safe irradiation dose of 4-10 kGy.After sterilization,the similarity between the fingerprint of Hedyotis diffusa and the control fingerprint was greater than 0.90.Liquid chromatography-mass spectrometry(LC-MS)confirmed the 14 compounds in the fingerprint spectrum.Grey correlation analysis(GRA)showed that the correlation degree r between the 14 compounds and the three antioxidant activities was greater than 0.5.Among them,peaks 8,10,and 11 had a reaction rate of over 50%with DPPH·.This study provided some scientific basis for the application of the electron beam technology in the production of Herba Hedyoti Diffusae.关键词
白花蛇舌草/电子束辐照灭菌/液质联用/抗氧化活性/多维度评价Key words
Herba Hedyoti Diffusae/electron beam sterilization/HPLC-MS/antioxidant activity/multidimensional evaluation引用本文复制引用
姜榃,于敏,焦连庆,田齐聪,庄新慧,姜先慧,田义新..多维度评价电子束辐照灭菌对白花蛇舌草质量的影响[J].核农学报,2024,38(10):1922-1929,8.基金项目
省级重点研发项目(2022C041-6) （2022C041-6）
