湖南中医药大学学报2024，Vol.44Issue(7)：1145-1151,7.DOI:10.3969/j.issn.1674-070X.2024.07.001
六味地黄汤调控TGF-β及Smad家族蛋白表达对肾间质纤维化的影响研究
Effects of Liuwei Dihuang Decoction on renal interstitial fibrosis by regulating the expressions of TGF-β and Smad family proteins
摘要
Abstract
Objective To explore the intervention effects and mechanism of Liuwei Dihuang Decoction(LWDHD)on inhibiting renal interstitial fibrosis(RIF).Methods A total of 30 SPF SD rats were randomly divided into sham-operated group,model group,positive drug group,high-and low-dose Chinese medicines groups,with six rats in each group.Except for the sham-operated group,all other groups were prepared with left ureteral ligation to establish RIF models.After modeling,the sham-operated group and model group were given distilled water by gavage,the positive drug group was given acetic acid prednisone tablets 0.2 mg/kg by gavage,and the high-and low-dose Chinese medicines groups were given LWDHD 1 mg/kg and 0.5 mg/kg by gavage,respectively,for a treatment course of 21 d.After treatment,renal interstitial injury was evaluated by HE staining,and renal interstitial collagen deposition and fibrosis were measured by Masson staining.The mRNA expressions of transforming growth factor-β1(TGF-β1),recombinant mothers against decapentaplegic homolog 2(Smad2),Smad3,and Smad7 were determined by RT-PCR.The protein expressions of TGF-β1,Smad2,Smad3,and Smad7 in the renal interstitium were determined by Western blot.Results Compared with the sham-operated group,the model group exhibited pathological features of mesangial proliferation,interstitial widening,and extensive collagen deposition and obvious fibrosis,as well as elevated mRNA and protein expressions of TGF-β1,Smad2,Smad3,and Smad7(P<0.05).Compared with the model group,the positive drug group,low-and high-dose Chinese medicines groups showed alleviated pathological changes,as well as a decrease in the mRNA and protein expressions of TGF-β1,Smad2,Smad3,and Smad7(P<0.05).Compared with the low-dose Chinese medicines group,the mRNA and protein expressions of TGF-β1,Smad2,Smad3,and Smad7 in the positive drug group and the high-dose Chinese medicines group decreased(P<0.05).Conclusion LWDHD can reduce renal interstitial fibrosis in RIF rats,with higher doses exhibiting more pronounced effects.The mechanism may be related to the regulation of TGF-β1 and Smad family protein expression.关键词
六味地黄汤/肾间质纤维化/终末期肾病/肾间质损伤/TGF-β/Smad家族蛋白Key words
Liuwei Dihuang Decoction/renal interstitial fibrosis/end-stage renal disease/renal interstitial injury/TGF-β/Smad family protein分类
医药卫生引用本文复制引用
蔡亚宏,刘林券,冯进,蔡喆燚,陈娟,李莎,熊丹..六味地黄汤调控TGF-β及Smad家族蛋白表达对肾间质纤维化的影响研究[J].湖南中医药大学学报,2024,44(7):1145-1151,7.基金项目
湖南省自然科学基金项目(2020JJ4474) （2020JJ4474）
湖南省卫生健康委员会科研计划项目(D202314018599,W20243021) （D202314018599,W20243021）
湖南中医药大学校院联合基金项目(2023XYLH044). （2023XYLH044）
