湖南中医药大学学报2024，Vol.44Issue(7)：1175-1180,6.DOI:10.3969/j.issn.1674-070X.2024.07.005
基于HMGB1炎症通路的苍耳子砂炒减毒机制研究
Toxicity reduction mechanism of Cangerzi(Xanthii Fructus)stir-fried with sand based on HMGB1 inflammatory pathway
摘要
Abstract
Objective To study the toxicity reduction mechanism of Cangerzi(Xanthii Fructus)stir-fried with sand based on high mobility group box protein B1(HMGB1)inflammatory pathway,and to provide experimental basis for the toxicity reducing mechanism of its processing.Methods KM male mice were randomized into normal,raw Cangerzi(Xanthii Fructus),and Cangerzi(Xanthii Fructus)stir-fried with sand groups.The raw Cangerzi(Xanthii Fructus)and Cangerzi(Xanthii Fructus)stir-fried with sand groups were given corresponding drugs by gavage,respectively,the normal group was administered with the same amount of normal saline by gavage,for 14 d continually.The serum alanine transaminase(ALT)and aspartate transaminase(AST)of mice were measured 1 h after administration on the 14th day.The body mass and liver coefficient of mice were tested.The liver histopathology of mice was observed after HE staining.The serum levels of inflammatory factors HMGB1,interleukin-1β(IL-1β),interleukin-2(IL-2),and tumor necrosis factor-α(TNF-α)of mice were determined by ELISA.Western blot and RT-qPCR were performed to test protein and mRNA expression levels of HMGB1,nuclear factor-κB(NF-κB),and Toll-like receptor 4(TLR4)in liver tissues of mice,respectively.Results After the administration,compared with the normal group,the serum values of AST and ALT of the raw Cangerzi(Xanthii Fructus)and Cangerzi(Xanthii Fructus)stir-fried with sand groups increased(P<0.05),the body mass decreased(P<0.05),the pathological structure of the liver was damaged,the liver index increased(P<0.05),and the serum levels of HMGB1,IL-1β,IL-2,and TNF-α were all higher(P<0.01).Meanwhile,the values of Cangerzi(Xanthii Fructus)stir-fried with sand group were lower than those of raw Cangerzi(Xanthii Fructus)group(P<0.01).Westen blot and RT-qPCR showed that the protein and mRNA expressions of HMGB1,TLR4,and NF-κB in liver tissue of the medication groups were all significantly higher(P<0.05),which were more obvious in the raw Cangerzi(Xanthii Fructus)group than those in the Cangerzi(Xanthii Fructus)stir-fried with sand group(P<0.05).Conclusion Cangerzi(Xanthii Fructus)has certain hepatotoxicity,and its toxicity was significantly reduced after being stir-fried with sand.The toxicity reduction mechanism may be related to the regulation of HMGB1-TLR4/(NF-κB)inflammatory pathway.关键词
苍耳子/砂炒法/肝毒性/HMGB1炎症通路/生物学机制Key words
Cangerzi(Xanthii Fructus)/method of stir-frying with sand/hepatotoxicity/high mobility group box protein B1 inflammatory pathway/biological mechanism分类
医药卫生引用本文复制引用
丁振涛,韩荣荣,余彦彤,黄成,黄保生,朋汤义,韩燕全..基于HMGB1炎症通路的苍耳子砂炒减毒机制研究[J].湖南中医药大学学报,2024,44(7):1175-1180,6.基金项目
国家重点研发计划项目(2023YFC3504202) （2023YFC3504202）
安徽省高校自然科学基金重点项目(2023AH050779) （2023AH050779）
安徽省名中药师工作室项目(皖财社[2023]23号) （皖财社[2023]23号）
安徽省中医药学术流派传承工作室建设项目(皖中医药发展秘[2021]30号). （皖中医药发展秘[2021]30号）
