湖南中医药大学学报2024，Vol.44Issue(7)：1193-1202,10.DOI:10.3969/j.issn.1674-070X.2024.07.007
基于指纹图谱的化学模式识别法和多指标测定评价枸杞不同部位的差异性
Evaluating the differences in different parts of Lycium barbarum L.by fingerprint-based chemical pattern recognition method and multi-index determination
摘要
Abstract
Objective To study the overall information and content differences of the main chemical components in different parts of Lycium barbarum L.,and comprehensively evaluate the quality and rational application of different parts of Lycium barbarum L..Methods This study focused on Gouqizi(Lycii Fructus),Gouqiye(Lycii leafy),and Digupi(Lycii Cortex)from three major producing regions as the subjects of investigation.HPLC fingerprints were established for these samples,and similarity evaluations were performed using the chromatographic fingerprint similarity evaluation system for Chinese medicines.Clustering and principal component analysis were conducted using SIMCA 14.1 software.The polysaccharide and total flavonoid contents were determined through spectrophotometric colorimetric methods,while the betaine content was quantified using the HPLC method.Results Significant differences were observed in the types and contents of chemical components among different parts of Lycium barbarum L.Gouqiye(Lycii leafy)and Gouqizi(Lycii Fructus)were found to contain richer chemical components.The contents of polysaccharides,total flavonoids,and betaine were ranked in the order of Gouqiye(Lycii leafy)＞Gouqizi(Lycii Fructus)＞Digupi(Lycii Cortex).Conclusion Gouqiye(Lycii leafy)are richer in nutrients,the pharmacological functions and nutritional value may be superior to those of Gouqizi(Lycii Fructus).Therefore,the multi-origin development of Gouqiye(Lycii leafy)as high-quality Lycium barbarum medicinal herbs can be considered for the multi-path and multi-level development and utilization of Lycium barbarum L.resources.关键词
枸杞子/枸杞叶/地骨皮/HPLC指纹图谱/化学计量学/多糖/黄酮/甜菜碱Key words
Lycii Fructus/Lycii leafy/Lycii Cortex/HPLC fingerprint/chemometrics/polysaccharides/flavonoids/betaine分类
医药卫生引用本文复制引用
闫艳,葛雨竹,张小倩,黄珊,赵权,王婕,杨晓帆..基于指纹图谱的化学模式识别法和多指标测定评价枸杞不同部位的差异性[J].湖南中医药大学学报,2024,44(7):1193-1202,10.基金项目
安徽省高校自然科学研究重点项目(2023AH052272,KJ2021A1149) （2023AH052272,KJ2021A1149）
亳州学院校级质量工程项目(2023XJXM134). （2023XJXM134）
