基于网络药理学及实验验证探讨补阳还五汤治疗脊髓损伤的作用机制OACSTPCD

Objective To investigate the potential targets and mechanisms of Buyang Huanwu Decoction(BYHWD)in the treatment of spinal cord injury through a combination of network pharmacology and experimental validation.Methods Relevant targets for BYHWD and spinal cord injury were screened through network pharmacology databases and mapped.Common targets were subjected to PPI network analysis and GO and KEGG enrichment analyses.A rat model of spinal cord injury was established,and the successfully modeled rats were randomized into sham-operated,model,and BYHWD groups to explore and validate the relevant pathways and targets of BYHWD in the treatment of spinal cord injury.Results A total of 214 BYHWD targets and 2,647 spinal cord injury-related targets were obtained,with 58 common targets intersected.PPI network analysis identified two core subnetworks,with ALB,Akt1,HIF-1α,VEGFA,and JUN as key targets.GO analysis yielded 1,583 entries.KEGG analysis indicated that the treatment of spinal cord injury with BYHWD was closely associated with multiple pathways,including PI3K-Akt,NF-κB,and P53.The Basso-Beattie-Bresnahan(BBB)score of the BYHWD group was higher than that of the model group(P<0.01,P<0.0001).BYHWD upregulated the levels of key proteins HIF-1α,ALB,VEGFA,and JUN after spinal cord injury(P<0.05,P<0.01),and downregulated the levels of pathway proteins p-PI3K/PI3K,p-Akt1/Akt1,p-NF-κB p65/NF-κB p65,and P53 after spinal cord injury(P<0.05).Conclusion BYHWD can inhibit the PI3K-Akt,NF-κB,and P53 pathways and promote the expressions of key proteins such as HIF-1α,ALB,VEGFA,and JUN in the injured segment after SD rat spinal cord injury,thereby promoting the recovery of neural function and exerting a therapeutic effect on spinal cord injury.