湖南中医药大学学报2024，Vol.44Issue(8)：1366-1372,7.DOI:10.3969/j.issn.1674-070X.2024.08.003
牛膝提取物含药血清对Aβ1-42诱导的神经元氧化应激和凋亡的调控机制研究
Regulatory mechanism of Achyranthis bidentatae Radix extract-containing serum on neuronal oxidative stress and apoptosis induced by Aβ1-42
摘要
Abstract
Objective To explore the effects and mechanism of Achyranthis bidentatae Radix extract-containing serum on neuronal oxidative stress and apoptosis induced by amyloid β-protein1-42(Aβ1-42).Methods Rat hippocampal neurons were cultured in vitro and divided into control group,model group(Aβ1-42 induced nerves),low-,medium-,and high-dose Achyranthis bidentatae Radix groups(15,30,45 μg/mL Achyranthis bidentatae Radix extract-containing serum on Aβ1-42-induced nerves),si-ZBTB20-AS1 group[Aβ1-42 induced neurons transfected with antisense RNA of zinc finger and BTB domain protein 20 small interfering RNA]and si-NC group(Aβ1-42 induced neurons transfected with disorderly meaningless negative sequence).Content of superoxide dismutase(SOD)and malondialdehyde(MDA)were determined by ELISA;CCK-8 method was used to check the cell survival rate;flow cytometry was used to test the apoptosis rate;RT-qPCR was used to check the expression level of ZBTB20-AS1;Western blot method was used to measure the expression levels of cell proliferation antigen Ki67 and cysteine aspartic acid-specific protease-3(Caspase-3)protein.Results Compared with control group,SOD content,cell survival rate,and the protein expression of Ki67 in model group were lower(P<0.05),MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were higher(P<0.05).Compared with the model group,SOD content,cell survival rate,and the protein expression of Ki67 in the middle-and high-dose Achyranthis bidentatae Radix extract groups were higher(P<0.05),and MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were lower(P<0.05).Compared with the si-NC group,SOD content,cell survival rate,and the protein expression of Ki67 in the si-ZBTB20-AS1 group were higher(P<0.05),and MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were lower(P<0.05).Conclusion Achyranthis bidentatae Radix extract-containing serum may inhibit Aβ1-42 induced oxidative stress and apoptosis of hippocampal neurons by down-regulating the expression of ZBTB20-AS1.关键词
牛膝提取物/β淀粉样蛋白1-42/海马神经元/氧化应激/凋亡Key words
Achyranthis bidentatae Radix extract/amyloid β-protein1-42/hippocampal neurons/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
马宝君,叶涵斌,孟高强,叶子..牛膝提取物含药血清对Aβ1-42诱导的神经元氧化应激和凋亡的调控机制研究[J].湖南中医药大学学报,2024,44(8):1366-1372,7.基金项目
江苏省自然科学基金项目(BK20221043). （BK20221043）
