湖南中医药大学学报2024，Vol.44Issue(8)：1385-1390,6.DOI:10.3969/j.issn.1674-070X.2024.08.005
一种新型负载淫羊藿苷的普鲁兰纳米粒制备及其性能研究
Preparation and performance study of a novel icariin-loaded pullulan nanoparticle
摘要
Abstract
Objective To design and preliminarily explore a novel icariin-modified pullulan polymer for efficient loading of icariin via chemical linkage and physical encapsulation,so as to prepare a nanospherical carrier with sustained release properties and investigate its performance.Methods Icariin was grafted onto hydrophilic pullulan via succinic anhydride as a linker arm to form icariin-modified pullulan polymers,and the end products were identified by Fourier infrared spectroscopy.The dialysis method was used to prepare icariin-loaded pullulan nanoparticles.The particle size and zeta potential of the blank and drug-loaded nanoparticles were measured,and their morphology was observed using transmission electron microscopy,and their in vitro drug release behavior was determined under different pH conditions.Results The nanoparticles had regular morphology,uniform size,and homogeneous dimensions.The potential of blank nanoparticles was+2.23 mV,and that of drug-loaded nanoparticles was+3.98 mV;the average particle size of blank nanoparticles was 122 nm,and that of drug-loaded nanoparticles was 190 nm.The nanoparticles could significantly prolong the sustained release time of epimedium glycosides,and it was more favorable for icariin release in the acidic condition of pH 6.8.Conclusion Nanoparticles with uniform size were successfully prepared by amphiphilic polymer self-assembly behavior.The Chinese medicine monomer icariin connected with pullulan was prepared as a nano-delivery system through the polymer chemical linkage mode of succinic acid,and the pullulan nano-delivery system loaded with icariin could make the hydrophobic drug into a nano type of water-soluble drug,and the drug had a slow-release and could be directed to be released under the environment of weak acidity.关键词
淫羊藿苷/普鲁兰多糖/纳米递送系统/药物释放/化学连接/缺血性心肌病Key words
icariin/pullulan polysaccharide/nanodelivery system/drug release/chemical connection/ischemic heart dis-ease分类
医药卫生引用本文复制引用
何艳娟,李湘,陈李凤,雷玉诚,贺宇康,曹志,邹登,邓发文,钟梓妮,袁立明..一种新型负载淫羊藿苷的普鲁兰纳米粒制备及其性能研究[J].湖南中医药大学学报,2024,44(8):1385-1390,6.基金项目
长沙市自然科学基金项目(kq2202021) （kq2202021）
湖南省大学生创新创业训练计划项目(S202212652014) （S202212652014）
湖南师范大学树达学院教学改革项目(院行发教务字[2022]35 号 6). （院行发教务字[2022]35 号 6）
