一种新型负载淫羊藿苷的普鲁兰纳米粒制备及其性能研究OACSTPCD

Objective To design and preliminarily explore a novel icariin-modified pullulan polymer for efficient loading of icariin via chemical linkage and physical encapsulation,so as to prepare a nanospherical carrier with sustained release properties and investigate its performance.Methods Icariin was grafted onto hydrophilic pullulan via succinic anhydride as a linker arm to form icariin-modified pullulan polymers,and the end products were identified by Fourier infrared spectroscopy.The dialysis method was used to prepare icariin-loaded pullulan nanoparticles.The particle size and zeta potential of the blank and drug-loaded nanoparticles were measured,and their morphology was observed using transmission electron microscopy,and their in vitro drug release behavior was determined under different pH conditions.Results The nanoparticles had regular morphology,uniform size,and homogeneous dimensions.The potential of blank nanoparticles was+2.23 mV,and that of drug-loaded nanoparticles was+3.98 mV;the average particle size of blank nanoparticles was 122 nm,and that of drug-loaded nanoparticles was 190 nm.The nanoparticles could significantly prolong the sustained release time of epimedium glycosides,and it was more favorable for icariin release in the acidic condition of pH 6.8.Conclusion Nanoparticles with uniform size were successfully prepared by amphiphilic polymer self-assembly behavior.The Chinese medicine monomer icariin connected with pullulan was prepared as a nano-delivery system through the polymer chemical linkage mode of succinic acid,and the pullulan nano-delivery system loaded with icariin could make the hydrophobic drug into a nano type of water-soluble drug,and the drug had a slow-release and could be directed to be released under the environment of weak acidity.