湖泊科学2024，Vol.36Issue(5)：1336-1352,中插1-中插3,20.DOI:10.18307/2024.0511
基于环境DNA技术和形态学鉴定的浮游植物多样性比较
Comparative study on the biodiversity of phytoplankton based on environmental DNA technology and morphological identification
摘要
Abstract
Phytoplankton is a crucial component of lake ecosystems,and understanding its species diversity and community compo-sition is essential for grasping the functionality of lake ecosystems.In recent years,environmental DNA(eDNA)technology has in-creasingly been applied in ecological surveys.However,whether the results based on eDNA technology are in line with those ob-tained through traditional morphological identification for detecting phytoplankton remains unclear.Additionally,there is a lack of research on simultaneously detecting both eukaryotic and prokaryotic phytoplankton communities using eDNA technology.In this study,we compared the results obtained by eDNA technology and morphological identification in detecting the species diversity and community composition of phytoplankton(including both eukaryotic and prokaryotic ones)in in-situ mesocosms exposed to different intensities of grazing pressure.The results indicated that:(1)eDNA technology based on the 18S rRNA gene could obtain diversity of prokaryotic phytoplankton community,the trends in phytoplankton alpha diversity(species diversity and Shannon-Wiener index)obtained by eDNA technology were consistent with those by morphological identification in the in-situ mesocosms;(2)Compared with the results of morphological identification,eDNA technology based on the 18S rRNA gene and 16S rRNA gene detected more species,effectively highlighting differences in community composition among different treatment groups;(3)eDNA technology based on the 18S rRNA gene demonstrated higher precision in detecting phytoplankton diversity.Despite limitations in monitoring phytoplankton biomass,eDNA technology exhibited significant advantages over morphological identification in assessing phyto-plankton biodiversity.This study attempted to apply eDNA technology to simultaneously detect both eukaryotic and prokaryotic phy-toplankton,it confirms the higher resolution and precision of eDNA technology in detecting phytoplankton species diversity and community composition,providing more comprehensive data for phytoplankton monitoring.This provides a basis for the broader a-doption of eDNA technology in the study of phytoplankton diversity and aquatic environmental monitoring.关键词
环境DNA/形态学鉴定/浮游植物/多样性Key words
Environmental DNA/morphological identification/phytoplankton/diversity引用本文复制引用
程云山,任艺晨,席贻龙,刘燕茹,何虎,范方威,陈东宜,李化炳..基于环境DNA技术和形态学鉴定的浮游植物多样性比较[J].湖泊科学,2024,36(5):1336-1352,中插1-中插3,20.基金项目
国家自然科学基金项目(32171535,31971471,32101321)、青藏高原第二次科学考察项目(2019QZKK0503)和中国科学院南京地理与湖泊研究所自主部署科研项目(NIGLAS2022GS02)联合资助. （32171535,31971471,32101321）
