裂片石莼水培液对海水养殖环境分离弧菌的抑制特性及其活性物质类型分析OA北大核心CSTPCD

Live macroalgae serve as an effective strategy to control pathogenic Vibrio(V.)pollution in mariculture,primarily due to the antibacterial active substances they secrete.However,limited information is available on the types of substances secreted by live macroalgae and their inhibitory performance on Vibrio in the mariculture environment.In this study,hydroponic solutions of Ulva fasciata(HSUF)were prepared at varying densities(1.25 g·L-1-10 g·L-1)and culture times(1-3 d).Six antibiotic-resistant Vibrio strains were isolated from the mariculture environment,including Vibrio parahaemolyticus(Vp.W1),Vibrio sinaloensis(Vs.W2),Vibrio metschnikovii(Vm.R3),and Vibrio alginolyticus(Va.W1,Va.S1,Va.M3).The structural composition of HSUF and their inhibitory performance against the isolated Vibrio strains were further analyzed.With the 12 h-culture of 3-d HSUF at different densities,the inhibi-tion rates were superior at 10 g·L-1.The inhibition rates of Vp.W1,Vs.W2,and Vm.R3 were significantly higher with 3-d HSUF(64％-100％)than with 1-d HSUF(1.32％-39.42％).However,there was no significant difference in inhibi-tion of Vibrio alginolyticus strains between 1-d and 3-d culture HSUF,with all inhibition rates being lower than 35％.Solid phase extraction-gas chromatography-mass spectrometry analysis revealed that HSUF contained a rich array of active substances,including acids,alcohol phenols,aldehydes and ketones,alkanes and aromatic hydrocarbons,het-erocycles,and esters.Moreover,four active substances(2,4,6-tribromophenol,2-(4-hydroxyphenyl)ethanol,acetal,and mono(2-Ethylhexyl)phthalate)were primarily found in HSUF.