江汉大学学报（自然科学版）2024，Vol.52Issue(4)：55-61,7.DOI:10.16389/j.cnki.cn42-1737/n.2024.04.006
红花中总生物碱回流提取工艺优化
Optimization of the Reflux Extraction Process of Total Alkaloids from Carthamus tinctorius
方振峰 1简德乾 1熊诗波1
作者信息
- 1. 江汉大学 医学部药学系,湖北 武汉 430056
- 折叠
摘要
Abstract
Objective To screen out the best reflux extraction process of total alkaloids from Carthamus tinctorius(C.tinctorius).Methods The effects of temperature,times,solid-liquid ratio,and methanol volume fraction on the extraction rate of alkaloids from C.tinctorius were investigated by a single-factor experiment with serotonin hydrochloride as reference substance and total alkaloid content of C.tinctorius as index.The extraction process of total alkaloids from C.tinctorius was optimized by an orthogonal experiment,and the verification experiment was carried out.Results The optimal process conditions were as follows:a temperature of 65℃,a liquid-to-material ratio(v/w)of 50∶1,a methanol volume fraction of 40%,and an extraction time of 60 minutes.Under optimized process conditions,the average content of total alkaloids was 35.99 mg/g with an RSD of 0.26% (n=3).Conclusion The optimal extraction process obtained in this study is simple and feasible,with reliable process parameters and good extraction results,providing an experimental basis for the further development and utilization of total alkaloids in C.tinctorius.关键词
红花/总生物碱/正交试验/提取工艺Key words
Carthamus tinctorius/total alkaloids/orthogonal test/extraction process分类
医药卫生引用本文复制引用
方振峰,简德乾,熊诗波..红花中总生物碱回流提取工艺优化[J].江汉大学学报（自然科学版）,2024,52(4):55-61,7.
