口腔颌面外科杂志2024，Vol.34Issue(4)：276-281,6.DOI:10.12439/kqhm.1005-4979.2024.04.004
索拉非尼抑制口腔鳞状细胞癌细胞的自噬、增殖及迁移
Sorafenib tosylate inhibits autophagy,proliferation and migration of oral squamous cell carcinoma
摘要
Abstract
Objective:To study the effect of Sorafenib tosylate(ST)on autophagy,proliferation and migration of CAL-27 cells in oral squamous cell carcinoma(OSCC).Methods:The CAL-27 cells were treated with different concentrations of ST,and their proliferation activity was detected by CCK-8 and cell clone formation assay;the effect of the CAL-27 cells migration was detected by cell wound scratch assay;the expressions of Beclin1,LC3B and PCNA were detected by western blotting.The CAL-27 cells were pretreated with the autophagy activator rapamycin,and the effects of ST on the proliferation,migration and expression of Beclin1,LC3B and PCNA in CAL-27 cells were detected after activation of autophagy.Results:Compared with the control group,ST significantly inhibited the proliferation and migration of the CAL-27 cells.ST decreased the expressions of Beclin-1,PCNA and increased the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B.However,when the CAL-27 cells were pretreated with RA to activate autophagy,the inhibitory effect of ST on proliferation and migration of the CAL-27 cells were weakened,the expressions of Beclin-1 and PCNA were increased,and the expressions of the cytoplasmic form LC3Ⅰ and the lipid form LC3Ⅱ of LC3B were decreased.Conclusion:ST may inhibit the proliferation and migration of CAL-27 cells by down-regulating Beclin-1,and inhibiting autophagy.Inhibition of autophagy may be a potential therapeutic strategy for OSCC.关键词
口腔鳞状细胞癌/自噬/增殖/迁移/索拉非尼Key words
oral squamous cell carcinoma/autophagy/proliferation/migration/Sorafenib tosylate分类
医药卫生引用本文复制引用
熊建哲,张昊,余伟..索拉非尼抑制口腔鳞状细胞癌细胞的自噬、增殖及迁移[J].口腔颌面外科杂志,2024,34(4):276-281,6.基金项目
十堰市科技局科研项目(21Y51) （21Y51）
