林产化学与工业2024，Vol.44Issue(4)：36-44,9.DOI:10.3969/j.issn.0253-2417.2024.04.005
无患子花总皂苷提取工艺优化及抗氧化、美白活性研究
Optimisation of Extraction Process of Sapindus mukorossi Gaertn.Flowers and in vitro Antioxidant and Whitening Activities
摘要
Abstract
Single-factor and orthogonal tests were used to optimize the extraction process of total saponins from Sapindus mukorossi Gaertn.flowers,using the total saponin yield as an indicator.The total saponins,flavonoids and phenols contents in the water extracts of S.mukorossi Gaertn.flowers and pericarp were compared.Furthermore,the antioxidant activity was evaluated by DPPH·,ABTS+·,O2-·radical scavenging test,and ferric ion reducing ability assay.The whitening effect was explored by tyrosinase activity inhibition test,and the irritant properties were investigated by red blood cell haemolysis test.The results showed that the optimum extraction conditions were as follows:extraction temperature of 80 ℃,extraction time of 180 min and liquid-solid ratio of 30(mL/g).Under these conditions,the total saponins yield was 12.15％.The contents of total saponins,flavonoids and phenols in the water extract of S.mukorossi Gaertn.flower were higher than that of pericarp.The antioxidant capacity and tyrosinase activity inhibiting ability of the S.mukorossi Gaertn.flower water extract were good,which were better than that of pericarp.The HD50/DI＞100 of the aqueous extract of S.mukorossi Gaertn.flowers,according to the erythrocyte haemolysis test irritation grading criteria,indicated that the aqueous extract was non-irritating.关键词
无患子花/皂苷/黄酮/抗氧化/酪氨酸酶Key words
Sapindus mukorossi Gaertn.flowers/saponins/flavonoids/antioxidant/tyrosinase分类
化学化工引用本文复制引用
张澳环,赵子冰,王晰,翁学煌,何华名..无患子花总皂苷提取工艺优化及抗氧化、美白活性研究[J].林产化学与工业,2024,44(4):36-44,9.基金项目
国家自然科学基金资助项目(32100254) （32100254）
2021年京明合作市级科技特派员专项项目(2021-N-24) （2021-N-24）
