林产化学与工业2024，Vol.44Issue(4)：70-78,9.DOI:10.3969/j.issn.0253-2417.2024.04.009
龙竹竹叶不同极性溶剂萃取物的抗氧化及体外降血糖和降血脂活性研究
Antioxidant,Hypoglycemic and Hypolipidemic Activities in vitro of Different Polar Solvent Extracts from Dendrocalamus giganteus Leaves
摘要
Abstract
The 70％ethanol extract from Dedrocalamus giganteus leaves was fractionated by successive extraction with petroleum ether,dichloromethane,ethyl acetate and n-butanol by graded extraction to obtain five different polar solvent extracts,including petroleum ether extract(PEE),dichloromethane extract(DME),ethyl acetate extract(EAE),n-butanol extract(BE),and water extract(WE),respectively.The contents of total flavonoids and polyphenols were determined,and the antioxidant,hypoglycemic and hypolipidemic activities of the five extracts in vitro were also evaluated.The results showed that the total flavonoid and polyphenols mass fractions of the ethyl acetate extract were the highest with(195.14±0.46)and(52.01±0.39)mg/g,respectively which were significantly higher than those of the other extracts(p＜0.05).Furthermore,all the different polar solvent extracts showed a certain antioxidant activity,hypoglycemic and hypolipidemic abilities in vitro.Among them,the ethyl acetate extract had the strongest scavenging activities on ABTS+·,inhibiting activities on α-glucosidase and α-amylase,and the half inhibitory concentration(ICs0)values were(118.73±0.29),(45.74±3.31)and(53.81±2.59)mg/L,respectively.Meanwhile,the binding rates of the ethyl acetate extract with sodium glycinate and sodium taurocholate were the highest at the same concentration,which reached(64.06±0.18)％and(59.27±0.35)％,at a mass concentration of 0.25 g/L,respectively.Polyphenol content of different extracts of D.giganteus leaves showed significant positive correlation with DPPH·and ABTS+·scavenging abilities(p＜0.05),with correlation coefficients of 0.94 and 0.81,respectively.The content of total flavonoids showed significant positive correlation with hypoglycemic and hypolipidemic ability in vitro(p＜0.05),with correlation coefficients were 0.75-0.84.关键词
龙竹竹叶/总黄酮/多酚/生物活性/相关性分析Key words
Dedrocalamus giganteus leaves/total flavonoids/polyphenols/biological activity/correlation analysis分类
化学化工引用本文复制引用
徐柠檬,资璐熙,李为兰,华燕,郭磊..龙竹竹叶不同极性溶剂萃取物的抗氧化及体外降血糖和降血脂活性研究[J].林产化学与工业,2024,44(4):70-78,9.基金项目
云南省科技厅农业联合专项(202101BD07001-054) （202101BD07001-054）
云南省科技厅基础研究计划(202101AT070042) （202101AT070042）
