葛根素在高糖条件下对大鼠足细胞自噬相关蛋白表达的影响OACSTPCD

Objective To observe the effects of puerarin on the autophagy pathway of silencing information regulator 1(SIRT1)/forkhead transcription factor O3(FOXO3)in rat podocytes under the condition of high glucose,and to explore the therapeutic effect of puerarin on diabetic kidney disease(DKD).Methods The expressions of autophagy-related proteins SIRT1,FOXO3a,p62 and Beclin-1 were detected by protein immunoblotting and immunofluorescence under high glucose con-dition.Results Beclin-1 expression in rat podocytes of high glucose group was down-regulated,p62 expression in podocytes was up-regulated,as compared with NG group,and SIRT1/FOXO3 autophagy pathway was inhibited.However,after the ap-plication of puerarin,Beclin-1 expression was up-regulated,p62 expression was down-regulated,and SIRT1/FOXO3 autoph-agy pathway was restored,showing significant difference(P＜0.05,P＜0.01).Conclusion Puerarin can reverse the inhi-bition of podocyte autophagy under high glucose condition,and can be expected to be a new drug for the treatment of DKD.