临床误诊误治2024，Vol.37Issue(14)：95-100,6.DOI:10.3969/j.issn.1002-3429.2024.14.020
葛根素在高糖条件下对大鼠足细胞自噬相关蛋白表达的影响
Effect of Puerarin on Autophagy-Related Protein Expression in Rat Podo-cytes Under High Glucose Condition
摘要
Abstract
Objective To observe the effects of puerarin on the autophagy pathway of silencing information regulator 1(SIRT1)/forkhead transcription factor O3(FOXO3)in rat podocytes under the condition of high glucose,and to explore the therapeutic effect of puerarin on diabetic kidney disease(DKD).Methods The expressions of autophagy-related proteins SIRT1,FOXO3a,p62 and Beclin-1 were detected by protein immunoblotting and immunofluorescence under high glucose con-dition.Results Beclin-1 expression in rat podocytes of high glucose group was down-regulated,p62 expression in podocytes was up-regulated,as compared with NG group,and SIRT1/FOXO3 autophagy pathway was inhibited.However,after the ap-plication of puerarin,Beclin-1 expression was up-regulated,p62 expression was down-regulated,and SIRT1/FOXO3 autoph-agy pathway was restored,showing significant difference(P＜0.05,P＜0.01).Conclusion Puerarin can reverse the inhi-bition of podocyte autophagy under high glucose condition,and can be expected to be a new drug for the treatment of DKD.关键词
糖尿病肾病/足细胞/大鼠/葛根素/高糖/自噬/沉默信息调节因子-1/叉头转录因子O3Key words
Diabetic kidney disease/Podocyte/Rat/Puerarin/High sugar/Autophagy/Silent information regulator factor-1/Forked transcription factor O3分类
医药卫生引用本文复制引用
陈亚巍,排尔哈提·凯赛尔,王士磊..葛根素在高糖条件下对大鼠足细胞自噬相关蛋白表达的影响[J].临床误诊误治,2024,37(14):95-100,6.基金项目
联保部队第九八三医院科技攻关课题(983YN22F05) （983YN22F05）
