老年医学与保健2024，Vol.30Issue(4)：1033-1040,8.
长链非编码RNALINC00184调控老年高血压血管内皮细胞的增殖与迁移
Regulation of proliferation and migration of vascular endothelial cells in elderly hypertension patients by long non-coding RNA LINC00184
摘要
Abstract
Objective Hypertension is an important pathogenic factor in the occurrence and development of atherosclero-sis in the elderly.The damage and aging of vascular endothelial cells accelerate the occurrence and development of atherosclero-sis.Long chain non-coding RNA(lncRNA)has been proved to play an important role in a variety of biological processes,but its role and molecular mechanism in hypertension-induced atherosclerosis and vascular endothelial damage are far from clear.Methods 51 elderly patients(≥60 years old)with complete medical history data from Department of Geriatrics,Renji Hospital from May 2019 to December 2020 were retrospectively collected.They were divided into three groups based on carotid intima-media thickness(CIMT)and blood pressure:hypertension with atherosclerosis group,hypertension without atherosclerosis group and atherosclerosis without hypertension group.The expression level of lncRNA in patients'blood was detected by fluorescence quantitative PCR.In human vascular endothelial cells,LINC00184 was knocked down by transfection of siRNA,and then the effects on the proliferation and migration of endothelial cells were assessed by morphological methods such as immunofluores-cence.The mechanism of LINC00184 involved in the regulation of vascular endothelial cells was determined by luciferase reporter gene assays and immunoblotting.Results There were significant differences in CIMT,systolic blood pressure and diastolic blood pressure among the three groups(P＜0.05).LINC00184 was highly expressed in plasma of elderly patients with hypertension.LINC00184 was highly expressed in human vascular endothelial cells and mainly distributed in the cytoplasm.Knockdown of LINC00184 could enhance the proliferation and migration of vascular endothelial cells.LINC00184 was involved in the function of vascular endothelial cells by influencing miR-17 to regulate the expression of PTEN.Conclusion LINC00184 is involved in the regulation of the proliferation and migration of vascular endothelial cells in elderly hypertension patients,which provides a theo-retical basis for further exploring the molecular mechanism of target organ damage caused by hypertension.关键词
老年/高血压/长链非编码RNA/血管内皮细胞/LINC00184Key words
elderly/hypertension/long non-coding RNA/endothelial cell/LINC00184引用本文复制引用
赵鑫,刘红霞,朱理安,胡耀敏..长链非编码RNALINC00184调控老年高血压血管内皮细胞的增殖与迁移[J].老年医学与保健,2024,30(4):1033-1040,8.基金项目
上海市卫生健康委员会/医疗保健项目(202140058) （202140058）
