内蒙古民族大学学报（自然科学版）2024，Vol.39Issue(4)：20-27,8.DOI:10.14045/j.cnki.15-1220.2024.04.004
铁死亡与早晚期颈动脉斑块免疫浸润性研究
Study on the Ferroptosis and Immune Infiltration of Early and Advanced Carotid Artery Plaques
摘要
Abstract
Exploring the correlation between ferroptosis and immune infiltration in early and advanced carotid artery plaques through bioinformatics methods,so as to identify the occurrence of early and advanced carotid artery plaques.Differential analysis of early and advanced carotid artery plaque sample datasets was performed,ferroptosis-related genes were obtained from the Ferrdb database,and the intersection of the two was taken as the shared key genes.Enrichment analysis of the shared key genes was conducted,hub genes were selected through the construction of a protein-protein interaction network(PPI),and immune infiltration analysis was performed.The results showed that differential analysis identified 7 key shared genes.GO enrichment analysis results showed that the shared key genes were crucial for the external apoptosis signaling pathway negatively regulated by death domain receptors,endolysosome and cysteine-type peptidase activity.KEGG enrichment analysis results indicated that the shared key genes were mainly enriched in ferroptosis,porphyrin metabolism,antigen processing and presentation signal pathways.By constructing PPI,the CTSB,CP,LGMN,HMOX1 were identified as hub genes.Receiver operating characteristic curve(ROC)and area under the curve(AUC)analysis results showed that the four hub genes had good diagnostic efficiency.Immune infiltration analysis revealed that macrophage M0,macrophage M2,and mast cells resting were significantly different(P＜0.01)in early and advanced carotid artery plaque samples;memory B cells,T cells regulation,dendritic cells activation,and γδT cells were significantly different(P＜0.05)in early and advanced carotid artery plaque samples.There was also a correlation between the four hub genes and immune cells.In conclusion,there is a certain correlation between ferroptosis and immune infiltration in early and advanced carotid artery plaques,and the four hub genes can serve as a reference for diagnosing early and advanced carotid artery plaques.关键词
生物信息学/颈动脉斑块/铁死亡/免疫浸润分析Key words
bioinformatics/carotid plaque/ferroptosis/immune infiltration analysis分类
医药卫生引用本文复制引用
孙永明,王文燕,邢晓冬,王晓萍,母茗含..铁死亡与早晚期颈动脉斑块免疫浸润性研究[J].内蒙古民族大学学报（自然科学版）,2024,39(4):20-27,8.基金项目
内蒙古自治区自然科学基金项目(2018LH08050) （2018LH08050）
内蒙古民族大学医养康复护理创新团队资助项目 （）
