品牌与标准化Issue(5)：1-3,3.DOI:10.3969/j.issn.1674-4977.2024.05.001
超高效液相色谱-串联质谱法测定黔产管花党参中党参炔苷含量
Determination of Lobetyolin Content in Codonopsis Tubulosa from Guizhou by UPLC-MS/MS
摘要
Abstract
Objective:To establish a method for determining the content of lobetyolin in Codonopsis tuberosa from Guizhou using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:The samples were extracted by ultrasonic extraction with 50%methanol.Under the conditions of acetonitrile-water(50∶50)as the mobile phase,a flow rate of 0.2 mL/min,and a column temperature of 35℃,the samples were separated on a Waters ACQUITY UPLC®BEH C18 column.The samples were ionized using an electrospray ionization source(ESI+)and quantitative analysis was performed using a standard curve method under multiple reaction monitoring(MRM)mode.Results:The linear relationship was good in the range of 0.10889 to 1.0889 ng,and the average recovery rate of spiked samples was 98.3%(n=6).Conclusion:This method is accurate and reliable,with high sensitivity and selectivity,and is suitable for the analysis,identification,and content determination of lobetyolin compounds in Codonopsis tuberosa from Guizhou.关键词
超高效液相色谱-串联质谱法/管花党参/党参炔苷/含量测定Key words
UPLC-MS/MS/codonopsis tubulosa/codonopsis lobetyolin/content determination引用本文复制引用
雷联伟,何羽,路利亚,张璐瑶..超高效液相色谱-串联质谱法测定黔产管花党参中党参炔苷含量[J].品牌与标准化,2024,(5):1-3,3.基金项目
毕节市科技创新平台及人才团队科技计划项目(毕科合[2023]16号):贵州党参(管花党参)质量控制方法研究. （毕科合[2023]16号）
